S100A4 Antibody (CL0237)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52890

Novus Biologicals, part of Bio-Techne
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NBP2-52890
NBP2-52890-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL0237

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all S100A4 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: MACPLEKALDVMVSTFHKYSGKEGDKFKLNKSELKELLTRELPSFLGKRTDEAAFQKLMSNLDSNRDNEVDFQEYCVFLSCIAMMCNEFFEGFPDKQPRKK

Epitope

KFKLNKSELKELLTR

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for S100A4 Antibody (CL0237)

Immunohistochemistry-Paraffin: S100A4 Antibody (CL0237) [NBP2-52890]

Immunohistochemistry-Paraffin: S100A4 Antibody (CL0237) [NBP2-52890]

Immunohistochemistry-Paraffin: S100A4 Antibody (CL0237) [NBP2-52890] - Staining in human tonsil and pancreas tissues. Corresponding S100A4 RNA-seq data are presented for the same tissues.
Western Blot: S100A4 Antibody (CL0237) [NBP2-52890]

Western Blot: S100A4 Antibody (CL0237) [NBP2-52890]

Western Blot: S100A4 Antibody (CL0237) [NBP2-52890] - Lane 1: Marker [kDa], Lane 2: Negative control (vector only transfected HEK293T lysate), Lane 3: S100A4 Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)
Immunocytochemistry/ Immunofluorescence: S100A4 Antibody (CL0237) [NBP2-52890]

Immunocytochemistry/ Immunofluorescence: S100A4 Antibody (CL0237) [NBP2-52890]

Immunocytochemistry/Immunofluorescence: S100A4 Antibody (CL0237) [NBP2-52890] - Staining of A549 cells using the Anti-S100A4 monoclonal antibody, showing specific staining in the plasma membrane and the cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
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Applications for S100A4 Antibody (CL0237)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Paraffin

1:2500 - 1:5000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Reviewed Applications

Read 1 review rated 4 using NBP2-52890 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: S100A4

S100A4 is encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Long Name

S100 Calcium Binding Protein A4

Alternate Names

18A2, 42A, CAPL, MTS1, P9KA, PEL98

Gene Symbol

S100A4

Additional S100A4 Products

Product Documents for S100A4 Antibody (CL0237)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for S100A4 Antibody (CL0237)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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