S100A4 Antibody (S100A4/1481)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-53178

Novus Biologicals, part of Bio-Techne
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NBP2-53178-100ug
NBP2-53178-20ug
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

ELISA, Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Western Blot

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # S100A4/1481

Concentration

0.2 mg/ml

View all S100A4 Antibodies »

Product Specifications

Immunogen

Recombinant fragment (around aa 1-200) of human S100A4 protein (exact sequence is proprietary) (Uniprot: P26447)

Localization

Nucleus, Cytoplasm

Marker

Marker of Tumor Metastasis

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54580)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for S100A4 Antibody (S100A4/1481)

Western Blot: S100A4 Antibody (S100A4/1481) [NBP2-53178]

Western Blot: S100A4 Antibody (S100A4/1481) [NBP2-53178]

Western Blot: S100A4 Antibody (S100A4/1481) [NBP2-53178] - Western Blot of HeLa, A549 and A375 Cell Lysate using S100A4 Monoclonal Antibody (S100A4/1481).
Immunohistochemistry: S100A4 Antibody (S100A4/1481) [NBP2-53178]

Immunohistochemistry: S100A4 Antibody (S100A4/1481) [NBP2-53178]

Immunohistochemistry: S100A4 Antibody (S100A4/1481) [NBP2-53178] - (A) CYR61 and/or S100A4 expression analysis in 123 invasive ductal carcinoma tissue sections and corresponding lymph node sections from 104 patients (B) and representative immunofluorescence staining analyzed with a 100x oil objective (Axio ZEISS). (C) Within the 123 invasive ductal carcinomas patient tissue sections 33 were stated as being TNBC. (D) Normal breast tissue sections (n = 8) were analyzed for their CYR61 and/ or S100A4 expression using immunofluorescence staining. Scale bar gauges 20 um. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fonc.2019.01074/full), licensed under a CC-BY license.
Western Blot: S100A4 Antibody (S100A4/1481) [NBP2-53178]

Western Blot: S100A4 Antibody (S100A4/1481) [NBP2-53178]

Western Blot: S100A4 Antibody (S100A4/1481) [NBP2-53178] - Western Blot Analysis of HeLa cell lysate using S100A4 Antibody (S100A4/1481).
View 5 more images

Applications for S100A4 Antibody (S100A4/1481)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: S100A4

S100A4 is encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Long Name

S100 Calcium Binding Protein A4

Alternate Names

18A2, 42A, CAPL, MTS1, P9KA, PEL98

Gene Symbol

S100A4

UniProt

P26447

Additional S100A4 Products

Product Documents for S100A4 Antibody (S100A4/1481)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for S100A4 Antibody (S100A4/1481)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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