S100A6 Antibody (JF0976)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-67123

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP2-67123
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JF0976

Concentration

1 mg/ml

View all S100A6 Antibodies »

Product Specifications

Immunogen

Recombinant full length protein of Human S100A6 aa 1-90 / 90. (SwissProt: P06703 Human; SwissProt: P14069 Mouse; SwissProt: P05964 Rat)

Localization

Nucleus envelope, Cytoplasm, Cell membrane.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for S100A6 Antibody (JF0976)

Western Blot: S100A6 Antibody (JF0976) [NBP2-67123]

Western Blot: S100A6 Antibody (JF0976) [NBP2-67123]

Western Blot: S100A6 Antibody (JF0976) [NBP2-67123] - Western blot analysis of S100A6 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A431 cell lysate Lane 2: mouse lung tissue lysate
Immunocytochemistry/ Immunofluorescence: S100A6 Antibody (JF0976) [NBP2-67123]

Immunocytochemistry/ Immunofluorescence: S100A6 Antibody (JF0976) [NBP2-67123]

Immunocytochemistry/Immunofluorescence: S100A6 Antibody (JF0976) [NBP2-67123] - Staining S100 alpha 6 in SH-SY-5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: S100A6 Antibody (JF0976) [NBP2-67123]

Immunohistochemistry-Paraffin: S100A6 Antibody (JF0976) [NBP2-67123]

Immunohistochemistry-Paraffin: S100A6 Antibody (JF0976) [NBP2-67123] - Analysis of paraffin-embedded mouse kidney tissue using anti-S100 alpha 6 antibody. Counter stained with hematoxylin.
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Applications for S100A6 Antibody (JF0976)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: S100A6

S100A6 is a member of the S100 family of proteins, and may function in stimulation of Ca2+-dependent insulin release, stimulation of prolactin secretion, and exocytosis. Recent studies have found that S100A6 is induced by tumour necrosis factor-alpha via a NF-kappaB-dependent mechanism, which serves a role in limiting tumour necrosis factor-alpha-induced apoptosis by regulating p53 phosphorylation.

Long Name

S100 Calcium Binding Protein A6

Alternate Names

CACY, MLN 4, PRA

Gene Symbol

S100A6

Additional S100A6 Products

Product Documents for S100A6 Antibody (JF0976)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for S100A6 Antibody (JF0976)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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