S100A8 Antibody (7C12/4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-51517

Novus Biologicals, part of Bio-Techne
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NBP1-51517-0.1ml
NBP1-51517-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Block/Neutralize, CyTOF-ready, ELISA, Flow Cytometry, Immunohistochemistry, Western Blot

Cited:

Block/Neutralize, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Clone # 7C12/4

Concentration

1 mg/ml

View all S100A8 Antibodies »

Product Specifications

Immunogen

Recombinant human S100A8

Clonality

Monoclonal

Host

Mouse

Isotype

IgM

Applications for S100A8 Antibody (7C12/4)

Application
Recommended Usage

ELISA

1:100 - 1:2000

Flow Cytometry

1:10 - 1:1000

Immunohistochemistry

1:10 - 1:500

Western Blot

1:100 - 1:2000
Application Notes
Positive control(s): Neutrophil lysate (from spleen). This antibody is Cytof ready. Use in blocking/ neutralizing reported in scientific literature (PMID: 11723110).

Formulation, Preparation, and Storage

Purification

Thiophilic Chromatography

Formulation

PBS

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: S100A8

The monoclonal antibody 7C12/4 recognizes an epitope on the S100A8 (MRP-8) protein. The calcium-binding, migration inhibitory factor-related proteins, MRP-8 (S100A8) and MRP-14 (S100A9) belong to the S100 protein family. The expression of these proteins is largely confined to the cytosol of neutrophils and monocytes. The complex formation of these proteins is a calcium-dependent process. The S100A8/A9 heterocomplex, also called MRP-8/MRP-14 complex or calprotectin, comprises 60% of the cytoplasmic protein fraction of circulating polymorphonuclear granulocytes and is also found in monocytes, macrophages and ileal tissue eosinophils. In inflammatory conditions small venueles stain with both anti-S100A8 and S100A9. The staining of the two subunits is always coincident. The S100A8/A9 complex has antibacterial, antifungal and immunomodulating and antiproliferative effects. Besides this it is a potent chemotactic factor for neutrophils.

Long Name

S100 Calcium Binding Protein A8

Alternate Names

Calgranulin A, CFAG, MRP8

Gene Symbol

S100A8

Additional S100A8 Products

Product Documents for S100A8 Antibody (7C12/4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for S100A8 Antibody (7C12/4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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