S100A8/S100A9 Heterodimer Antibody (27E10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-60157

Novus Biologicals, part of Bio-Techne
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NBP1-60157-0.05mg
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Key Product Details

Species Reactivity

Validated:

Human, Monkey

Cited:

Human

Applications

Validated:

Flow Cytometry, Immunoassay, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

IF/IHC, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 27E10

Concentration

0.1 mg/ml

View all S100A8/S100A9 Heterodimer Antibodies »

Product Specifications

Immunogen

Human blood monocytes

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for S100A8/S100A9 Heterodimer Antibody (27E10)

Immunoassay: S100A8/S100A9 Heterodimer Antibody (27E10) [NBP1-60157]

Immunoassay: S100A8/S100A9 Heterodimer Antibody (27E10) [NBP1-60157]

Immunoassay: S100A8/A9 Antibody (27E10) [NBP1-60157] - Immunoassay: S100A8/A9 Antibody (27E10) [NBP2-62801] Image using the Azide and BSA Free form of this antibody.

Applications for S100A8/S100A9 Heterodimer Antibody (27E10)

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
W: non-reduced;~22 kDa; doesn't recognize the single proteins S100A8 and S100A9. For IHC-F: acetone fixation; 0.1 % hydrogen peroxide treatment to reduce enogenous peroxidise activity; positive control: inflammatory tissue; negative control: normal human tissue (skin, lung, colon).

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.2 um filtered solution in PBS and 0.1% BSA

Preservative

0.02% Sodium Azide

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: S100A8/S100A9 Heterodimer

The monoclonal antibody 27E10 recognizes an epitope specific for the S100A8/A9 heterocomplex that is not exposed on the individual subunits S100A8 (MRP8, calgranulin-A) or S100A9 (MRP14, calgranulin-B). The calcium-binding migration inhibitory factor-related proteins, MRP-8 (S100A8) and MRP-14 (S100A9) belong to the S100 protein family. The expression of these proteins is largely confined to the cytosol of neutrophils and monocytes. The complex formation of these proteins is a calcium-dependent process. The S100A8/A9 heterocomplex, also called MRP-8/MRP-14 complex or calprotectin, comprises 60% of the cytoplasmic protein fraction of circulating polymorphonuclear granulocytes and is also found in monocytes, macrophages and ileal tissue eosinophils. Peripheral blood monocytes carry the antigen extra- and intracellularly, neutrophils only intracellularly. The S100A8/A9 complex has antibacterial, antifungal, immunomodulating and antiproliferative effects. Besides this it is a potent chemotactic factor for neutrophils. Plasma concentrations are elevated in diseases associated with increased neutrophil activity, like inflammatory bowel disease. Granulocytes terminate their existence after transmigration through the intestinal wall. Therefore calprotectin is also detectable in feces. Elevated levels of calprotectin have been observed in body fluids such as plasma, saliva, gingival crevicular fluid, stools, and synovial fluid during infection and inflammatory conditions. The monoclonal antibody 27E10 can be used for early detection of inflammatory macrophages, for the characterization of tumorous tissues and the monitoring of peripheral blood cell cultures. The antibody 27E10 does not react with lymphocytes or platelets.

Long Name

S100 Calcium Binding Protein A8/S100 Calcium Binding Protein A9

Alternate Names

Calprotectin

Gene Symbol

S100A8

Additional S100A8/S100A9 Heterodimer Products

Product Documents for S100A8/S100A9 Heterodimer Antibody (27E10)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for S100A8/S100A9 Heterodimer Antibody (27E10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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