S100A9 Antibody (47-8D3 (DF3973)) [DyLight 755]

Validated:

DyLight 755 (Excitation = 754 nm, Emission = 776 nm)

Monoclonal Mouse IgG₁ kappa Clone # 47-8D3 (DF3973)

Please see the vial label for concentration. If unlisted please contact technical services.

Human peripheral blood monocyte components (Uniprot: P06702)

Use in Mouse reported in scientific literature (PMID:32191647).

Cytoplasmic, Extracellular (secreted) and Cell Surface

This monoclonal antibody stains the cytoplasm of macrophages and histiocytes in hematopoietic organs, Kupffers cells of the skin. It also stains the mantle zone B-lymphocytes of the lymph node and spleen, spermatogonia, and chief cells of the stomach. S100A9 is expressed by macrophages in acutely inflamed tissues and in chronic inflammation. It is detected in peripheral blood leukocytes, in neutrophils and granulocytes. It is present at sites of vascular inflammation. S100A9 is also expressed in epithelial cells constitutively or induced during dermatoses. S100A9 is a Calcium-binding protein. It has antimicrobial activity towards bacteria and fungi. It is important for resistance to invasion by pathogenic bacteria. It up-regulates transcription of genes that are under the control of NF-kappa-B. S100A9 plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS). It promotes tubulin polymerization when unphosphorylated. It also promotes phagocyte migration and infiltration of granulocytes at sites of wounding. It plays a role as a pro-inflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1.

Macrophage Marker

Monoclonal

Mouse

IgG₁ kappa

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Protein A or G purified

50mM Sodium Borate

0.05% Sodium Azide

Please see the vial label for concentration. If unlisted please contact technical services.

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Store at 4C in the dark.