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S1P3/EDG-3 Antibody (5E3G9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15479

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-15479-100ul
NBP3-15479-20ul

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5E3G9

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human S1P3/EDG-3 (Q99500). MATALPPRLQPVRGNETLREHYQYVGKLAGRLKEASEGSTLTTVLFLVICSFIVLENLMVLIAIWKNNKFHNRMYFFIGNLALCDLLAGIAYKVNILMSG

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for S1P3/EDG-3 Antibody (5E3G9)

Western Blot: S1P3/EDG-3 Antibody (5E3G9) [NBP3-15479]

Western Blot: S1P3/EDG-3 Antibody (5E3G9) [NBP3-15479]

Western Blot: S1P3/EDG-3 Antibody (5E3G9) [NBP3-15479] - Western blot analysis of extracts of various cell lines, using S1P3/EDG-3 Rabbit mAb (NBP3-15479) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Applications for S1P3/EDG-3 Antibody (5E3G9)

Application
Recommended Usage

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: S1P3/EDG-3

EDG3, a Lysophospholipid/Lysosphingolipid Receptor, binds the vasoconstrictor sphingosine-1-phosphate and activates transcription of serum response element-driven reporter genes. EDG3 may regulate angiogenesis and vascular endothelial cell functions. EDG3 has been reported in all tissues, with highest levels in heart, placenta, kidney, and liver. ESTs have been isolated from placenta and germ cell tumor libraries.

Long Name

Endothelial Differentiation Gene 3

Alternate Names

EDG3, LPB3, S1PR3

Gene Symbol

S1PR3

Additional S1P3/EDG-3 Products

Product Documents for S1P3/EDG-3 Antibody (5E3G9)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for S1P3/EDG-3 Antibody (5E3G9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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