Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey, Primate

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI4A3

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all SAE2 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human UBA2 (NP_005490) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

71 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SAE2 Antibody (OTI4A3) - Azide and BSA Free

Western Blot: SAE2 Antibody (OTI4A3)Azide and BSA Free [NBP2-73973]

Western Blot: SAE2 Antibody (OTI4A3)Azide and BSA Free [NBP2-73973]

Western Blot: SAE2 Antibody (OTI4A3) - Azide and BSA Free [NBP2-73973] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SAE2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SAE2.
Immunocytochemistry/ Immunofluorescence: SAE2 Antibody (OTI4A3) - Azide and BSA Free [NBP2-73973]

Immunocytochemistry/ Immunofluorescence: SAE2 Antibody (OTI4A3) - Azide and BSA Free [NBP2-73973]

Immunocytochemistry/Immunofluorescence: SAE2 Antibody (OTI4A3) - Azide and BSA Free [NBP2-73973] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SAE2.
Immunohistochemistry: SAE2 Antibody (OTI4A3) - Azide and BSA Free [NBP2-73973]

Immunohistochemistry: SAE2 Antibody (OTI4A3) - Azide and BSA Free [NBP2-73973]

Immunohistochemistry: SAE2 Antibody (OTI4A3) - Azide and BSA Free [NBP2-73973] - Staining of paraffin-embedded Human lymphoma tissue using anti-SAE2 mouse monoclonal antibody.
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Applications for SAE2 Antibody (OTI4A3) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SAE2

Covalent attachment of one protein to another is one of the more prominent posttranslational modifications in respect to size and ubiquity. Ubiquitin is the most familiar of the protein modifiers and its activation and transfer to target proteins has been studied extensively. Recently, a new group of ubiquitin-like proteins has been receiving a lot of attention. SUMO, or Sentrin, is one of the most intriguing. SUMO has been implicated in the stabilization of target proteins and/or their localization to sub-cellular complexes. The conjugation of SUMO-1, SUMO-2 and SUMO-3 onto target proteins requires the concerted action of the specific E1-activating enzyme SAE1/SAE2, the E2-conjugating enzyme Ubc9, and an E3-like SUMO ligase.

Alternate Names

Anthracycline-associated resistance ARX, ARX, EC 6.3.2, EC 6.3.2.-, FLJ13058, HRIHFB2115, SAE2SUMO-1 activating enzyme subunit 2, SUMO1 activating enzyme subunit 2, SUMO-activating enzyme subunit 2, UBA2, ubiquitin-activating enzyme E1 homolog, Ubiquitin-like 1-activating enzyme E1B, ubiquitin-like modifier activating enzyme 2, UBLE1B

Gene Symbol

UBA2

Additional SAE2 Products

Product Documents for SAE2 Antibody (OTI4A3) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SAE2 Antibody (OTI4A3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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