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SAE2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-49685

Novus Biologicals, part of Bio-Techne
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NBP1-49685

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 590 and 640 of human SUMO1 Activating Enzyme Subunit 2 using the numbering given in entry NP_005490.1 (GeneID 10054).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SAE2 Antibody

SAE2 Antibody [NBP1-49685] - Detection of human and mouse SAE2 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-SAE2 antibody NBP1-49685 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. SAE2 was also immunoprecipitated by rabbit anti-SAE2 antibody NBP1-49684, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 1 second (B).
SAE2 Antibody [NBP1-49685] - Detection of human and mouse SAE2 by immunohistochemistry. Sample: FFPE sections of human prostate carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti- SAE2 (NBP1-49685) used at a dilution of 1:200 (1ug/ml). Detection: DAB

Applications for SAE2 Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 5 mcg/mg

Western Blot

1:2000 - 1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SAE2

The human UBiquitin Activating domain 2 (UBA2) gene is located on chromosome 19q12. Human UBA2 could form a beta mercaptoethanol-sensitive conjugate with members of the sentrin family, but not with ubiquitin of NEDD8 in the presence of AOS1. The recombinant proteins, Sua1p and hUba2, form a complex. In this complex, hUba2 binds SUMO-1, which gives the complex the activity of the SUMO-1 activating enzyme. Therefore, UBA2 has been found to be a component of the sentrin-activating enzyme complex.

Alternate Names

Anthracycline-associated resistance ARX, ARX, EC 6.3.2, EC 6.3.2.-, FLJ13058, HRIHFB2115, SAE2SUMO-1 activating enzyme subunit 2, SUMO1 activating enzyme subunit 2, SUMO-activating enzyme subunit 2, UBA2, ubiquitin-activating enzyme E1 homolog, Ubiquitin-like 1-activating enzyme E1B, ubiquitin-like modifier activating enzyme 2, UBLE1B

Gene Symbol

UBA2

Additional SAE2 Products

Product Documents for SAE2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SAE2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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