SAFB Antibody (5A11)

Novus Biologicals, part of Bio-Techne | Catalog # H00006294-M04

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 5A11

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SAFB Antibodies »

Product Specifications

Immunogen

SAFB (NP_002958, 111 a.a. ~ 200 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DGQEDVETSLENLQDIDIMDISVLDEAEIDNGSVADCVEDDDADNLQESLSDSRELVEGEMKELPEQLQEHAIEDKETINNLDTSSSDFT

Specificity

SAFB (5A11)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for SAFB Antibody (5A11)

Western Blot: SAFB Antibody (5A11) [H00006294-M04]

Western Blot: SAFB Antibody (5A11) [H00006294-M04]

Western Blot: SAFB Antibody (5A11) [H00006294-M04] - Analysis of SAFB expression in Hela S3 NE (Cat # L013V3).
Immunocytochemistry/ Immunofluorescence: SAFB Antibody (5A11) [H00006294-M04]

Immunocytochemistry/ Immunofluorescence: SAFB Antibody (5A11) [H00006294-M04]

Immunocytochemistry/Immunofluorescence: SAFB Antibody (5A11) [H00006294-M04] - Analysis of monoclonal antibody to SAFB on HeLa cell. Antibody concentration 10 ug/ml
Immunohistochemistry-Paraffin: SAFB Antibody (5A11) [H00006294-M04]

Immunohistochemistry-Paraffin: SAFB Antibody (5A11) [H00006294-M04]

Immunohistochemistry-Paraffin: SAFB Antibody (5A11) [H00006294-M04] - Analysis of monoclonal antibody to SAFB on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration 3 ug/ml
View 1 more images

Applications for SAFB Antibody (5A11)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SAFB

This gene encodes a DNA-binding protein that has high specificity for scaffold or matrix attachment region DNA elements (S/MAR DNA). This protein is thought to be involved in attaching the base of chromatin loops to the nuclear matrix but there is conflicting evidence as to whether this protein is a component of chromatin or a nuclear matrix protein. Scaffold attachment factors are a specific subset of nuclear matrix proteins (NMP) that specifically bind to S/MAR. This encoded protein is thought to serve as a molecular base to assemble a 'transcriptosome complex' in the vicinity of actively transcribed genes. It is involved in the regulation of the heat shock protein 27 transcription and also can act as an estrogen receptor corepressor. This gene is a candidate gene for breast tumorigenesis.

Alternate Names

glutathione S-transferase fusion protein, HAP, heat-shock protein (HSP27) estrogen response element and TATA box-bindingprotein, HETDKFZp779C1727, Hsp27 ERE-TATA binding protein, HSP27 ERE-TATA-binding protein, HSP27 estrogen response element-TATA box-binding protein, SAF-B, SAF-B1, SAFB1SAB-B1, scaffold attachment factor B, scaffold attachment factor B1

Entrez Gene IDs

6294 (Human)

Gene Symbol

SAFB

OMIM

602895 (Human)

UniProt

NP_002958 (Human)

Additional SAFB Products

Product Documents for SAFB Antibody (5A11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SAFB Antibody (5A11)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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