Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all SAH3 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 50 and 100 of human adenosylhomocysteinase 3 (KIAA0828) using the numbering given in entry NP_056143.1 (GeneID 23382).

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SAH3 Antibody

Western Blot: SAH3 Antibody [NB100-401]

Western Blot: SAH3 Antibody [NB100-401]

Western Blot: SAH3 Antibody [NB100-401] - Samples: NETN whole cell lysate (20 ug for WB; 1 mg for IP) from HeLa cells. Antibody: Affinity purified rabbit anti-SAH3 antibody used at the indicated concentration for WB and 2 ug/mg lysate for IP. Detection: HRP conjugated goat anti-rabbit IgG used at 1:10,000 followed by a chemiluminescent substrate and exposure to film for 10 seconds.
Immunocytochemistry/ Immunofluorescence: SAH3 Antibody [NB100-401]

Immunocytochemistry/ Immunofluorescence: SAH3 Antibody [NB100-401]

Immunocytochemistry/Immunofluorescence: SAH3 Antibody [NB100-401] - SAH3 antibody was tested in Neuro-2a cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 549 (red).

Applications for SAH3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunoprecipitation

1-4 ug/mg of lysate

Western Blot

1:1000-1:10000
Application Notes
This SAH3 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunoprecipitation, and Western Blot, where a band should be seen at ~70-80 kDa. In ICC/IF, cytoplasmic staining was observed in neuro-2a cells.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SAH3

In mammals, S-Adenosylhomocysteine hydrolase (SAH3 / AdoHcyase) catalyzes the hydrolysis of S-adenosylhomocysteine to form adenosine and homocysteine. AdoHcy is the product of all adenosylmethionine (AdoMet)-dependent biological transmethylations. These reactions have a wide range of products, and are common in all facets of biometabolism. As a product inhibitor, elevated levels of AdoHcy suppress AdoMet-dependent transmethylations. Thus, SAH3 is a regulator of biological transmethylation.

Alternate Names

adenosylhomocysteinase-like 2, AdoHcyase 3, ADOHCYASE3, EC 3.3.1.1, FLJ21719, KIAA0828S-adenosylhomocysteine hydrolase-like 2, putative adenosylhomocysteinase 3, S-adenosylhomocysteine hydrolase-like protein 2, S-adenosyl-L-homocysteine hydrolase 3

Entrez Gene IDs

23382 (Human)

Gene Symbol

AHCYL2

UniProt

NP_056143.1 (Human)

Additional SAH3 Products

Product Documents for SAH3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SAH3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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