SAH3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47293

Novus Biologicals, part of Bio-Techne
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NBP2-47293
NBP2-47293-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Rat (98%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SAH3 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: GKVPQASAMKRSDPHHQHQRHRDGGEALVSPDGTVTEAPRTVKKQIQFADQKQEFNKRPTKI

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 27896923).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SAH3 Antibody

Immunohistochemistry-Paraffin: SAH3 Antibody [NBP2-47293]

Immunohistochemistry-Paraffin: SAH3 Antibody [NBP2-47293]

Immunohistochemistry-Paraffin: SAH3 Antibody [NBP2-47293] - Analysis in human stomach and liver tissues. Corresponding AHCYL2 RNA-seq data are presented for the same tissues.
Western Blot: SAH3 Antibody [NBP2-47293]

Western Blot: SAH3 Antibody [NBP2-47293]

Western Blot: SAH3 Antibody [NBP2-47293] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG.
Immunocytochemistry/ Immunofluorescence: SAH3 Antibody [NBP2-47293]

Immunocytochemistry/ Immunofluorescence: SAH3 Antibody [NBP2-47293]

Immunocytochemistry/Immunofluorescence: SAH3 Antibody [NBP2-47293] - Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm & cytosol.
View 4 more images

Applications for SAH3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SAH3

In mammals, S-Adenosylhomocysteine hydrolase (SAH3 / AdoHcyase) catalyzes the hydrolysis of S-adenosylhomocysteine to form adenosine and homocysteine. AdoHcy is the product of all adenosylmethionine (AdoMet)-dependent biological transmethylations. These reactions have a wide range of products, and are common in all facets of biometabolism. As a product inhibitor, elevated levels of AdoHcy suppress AdoMet-dependent transmethylations. Thus, SAH3 is a regulator of biological transmethylation.

Alternate Names

adenosylhomocysteinase-like 2, AdoHcyase 3, ADOHCYASE3, EC 3.3.1.1, FLJ21719, KIAA0828S-adenosylhomocysteine hydrolase-like 2, putative adenosylhomocysteinase 3, S-adenosylhomocysteine hydrolase-like protein 2, S-adenosyl-L-homocysteine hydrolase 3

Gene Symbol

AHCYL2

Additional SAH3 Products

Product Documents for SAH3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SAH3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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