Salmonella Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB600-1087

Novus Biologicals, part of Bio-Techne
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NB600-1087
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Key Product Details

Species Reactivity

Validated:

Mouse, Bacteria

Cited:

Human, Bacteria

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

4.5 mg/ml

View all Salmonella Antibodies »

Product Specifications

Immunogen

Mixture of S. enteriditis, S. typhimurium, and S. heidelburg

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 27748732).

Localization

whole cell

Specificity

This is polyvalent for Salmonella "O" & "H" antigens. Immunocaptures Salmonellae. Antiserum is not absorbed and does react with related Enterobacteriaceae.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for Salmonella Antibody

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500
Application Notes
ELISA, Immunofluorescence. May be used in place of neat antiserum in almost any appropriate antibody-based technique. Immunocaptures Salmonellae. Optimal dilutions and concentrations should be determined by the end user. Purity is >95% Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 23732169)

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.01 M Phosphate Buffered Saline, pH 7.2

Preservative

0.1% Sodium Azide

Concentration

4.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Salmonella

The genus Salmonella is a member of the family Enterobacteriaceae. The genus is composed of Gram negative bacilli that are facultative and flagellated (motile). Salmonellae possess 3 major surface antigens; the H or flagellar antigen (phase 1 and 2), the O or somatic antigen (part of the LPS moiety) and the Vi or capsular antigen (referred to as K in other Enterobacteriaceae). Salmonellae also possess the LPS endotoxin characteristic of Gram negative bacteria. This LPS is composed of an O polysaccharide (O antigen) an R core and the endotoxic inner Lipid A. Endotoxins evoke fever and can activate complement, kinin and clotting factors.

Alternate Names

Salmonellae

Additional Salmonella Products

Product Documents for Salmonella Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Salmonella Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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