SAMHD1 Antibody (OTI3F5) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-73979

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey, Primate

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI3F5

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all SAMHD1 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human SAMHD1(NP_056289) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

72 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SAMHD1 Antibody (OTI3F5) - Azide and BSA Free

Western Blot: SAMHD1 Antibody (OTI3F5)Azide and BSA Free [NBP2-73979]

Western Blot: SAMHD1 Antibody (OTI3F5)Azide and BSA Free [NBP2-73979]

Western Blot: SAMHD1 Antibody (OTI3F5) - Azide and BSA Free [NBP2-73979] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SAMHD1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SAMHD1.
Immunocytochemistry/ Immunofluorescence: SAMHD1 Antibody (OTI3F5) - Azide and BSA Free [NBP2-73979]

Immunocytochemistry/ Immunofluorescence: SAMHD1 Antibody (OTI3F5) - Azide and BSA Free [NBP2-73979]

Immunocytochemistry/Immunofluorescence: SAMHD1 Antibody (OTI3F5) - Azide and BSA Free [NBP2-73979] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SAMHD1.
Immunohistochemistry: SAMHD1 Antibody (OTI3F5) - Azide and BSA Free [NBP2-73979]

Immunohistochemistry: SAMHD1 Antibody (OTI3F5) - Azide and BSA Free [NBP2-73979]

Immunohistochemistry: SAMHD1 Antibody (OTI3F5) - Azide and BSA Free [NBP2-73979] - Staining of paraffin-embedded Human pancreas tissue using anti-SAMHD1 mouse monoclonal antibody.
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Applications for SAMHD1 Antibody (OTI3F5) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:200-500

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SAMHD1

Putative nuclease involved in innate immune response by acting as a negative regulator of the cell-intrinsic antiviral response. May play a role in mediating proinflammatory responses to TNF-alpha signaling

Long Name

SAM Domain and HD Domain 1

Alternate Names

AGS5, CHBL2, DCIP, HDDC1, MOP-5, SBBI88

Gene Symbol

SAMHD1

Additional SAMHD1 Products

Product Documents for SAMHD1 Antibody (OTI3F5) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SAMHD1 Antibody (OTI3F5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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