SAP130 Antibody (2F1)

Novus Biologicals, part of Bio-Techne | Catalog # H00023450-M03

Novus Biologicals, part of Bio-Techne
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H00023450-M03
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2F1

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SAP130 Antibodies »

Product Specifications

Immunogen

SF3B3 (NP_036558.3, 819 a.a. ~ 929 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAEEMVEAAGEDERELAAEMAAAFLNENLPESIFGAPKAGNGQWASVIRVMNPIQGNTLDLVQLEQNEAAFSVAVCRFSNTGEDWYVLVGVAKDLILNPRSVAGGFVYTYK

Specificity

SF3B3 - splicing factor 3b, subunit 3, 130kDa (2F1)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for SAP130 Antibody (2F1)

Immunocytochemistry/ Immunofluorescence: SAP130 Antibody (2F1) [H00023450-M03]

Immunocytochemistry/ Immunofluorescence: SAP130 Antibody (2F1) [H00023450-M03]

Immunocytochemistry/Immunofluorescence: SAP130 Antibody (2F1) [H00023450-M03] - Immunofluorescence of monoclonal antibody to SAP130 on HeLa cell. [antibody concentration 40 ug/ml]
Immunocytochemistry/ Immunofluorescence: SAP130 Antibody (2F1) [H00023450-M03]

Immunocytochemistry/ Immunofluorescence: SAP130 Antibody (2F1) [H00023450-M03]

Immunocytochemistry/Immunofluorescence: SAP130 Antibody (2F1) [H00023450-M03] - Analysis of monoclonal antibody to SF3B3 on HeLa cell. Antibody concentration 40 ug/ml
Sandwich ELISA: SAP130 Antibody (2F1) [H00023450-M03] - Detection limit for recombinant GST tagged SF3B3 is 0.1 ng/ml as a capture antibody.

Applications for SAP130 Antibody (2F1)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This product is useful for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SAP130

This gene encodes subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 3 has also been identified as a component of the STAGA (SPT3-TAF(II)31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF(II)-containing complex). These complexes may function in chromatin modification, transcription, splicing, and DNA repair. [provided by RefSeq]

Alternate Names

KIAA0017splicing factor 3b, subunit 3, 130kD, Pre-mRNA-splicing factor SF3b 130 kDa subunit, RSE1, SAP130SAP 130, SF3B130, SF3b130pre-mRNA splicing factor SF3b, 130 kDa subunit, Spliceosome-associated protein 130, splicing factor 3B subunit 3, splicing factor 3b, subunit 3, 130kDa, STAF130

Entrez Gene IDs

23450 (Human)

Gene Symbol

SF3B3

UniProt

NP_036558 (Human)

Additional SAP130 Products

Product Documents for SAP130 Antibody (2F1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SAP130 Antibody (2F1)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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