SAP130 Antibody (S07-2K8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-19974

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-19974-100ul
NBP3-19974-50ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S07-2K8

Concentration

0.3 mg/ml

View all SAP130 Antibodies »

Product Specifications

Immunogen

A synthetic peptide of human SAP130 (Uniprot # Q15393)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

136 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SAP130 Antibody (S07-2K8)

Western Blot: SAP130 Antibody (S07-2K8) [NBP3-19974]

Western Blot: SAP130 Antibody (S07-2K8) [NBP3-19974]

Western Blot: SAP130 Antibody (S07-2K8) [NBP3-19974] - Western blot detection of SAP130 in SHSY-5Y, C6 cell lysates using NBP3-19974 (1:1000 diluted). Predicted band size: 136KDa. Observed band size: 136KDa.
Immunocytochemistry/ Immunofluorescence: SAP130 Antibody (S07-2K8) [NBP3-19974]

Immunocytochemistry/ Immunofluorescence: SAP130 Antibody (S07-2K8) [NBP3-19974]

Immunocytochemistry/Immunofluorescence: SAP130 Antibody (S07-2K8) [NBP3-19974] - Immunocytochemistry of SAP130 (green) in Hela cells using NBP3-19974 at dilution 1/50, and DAPI (blue)

Applications for SAP130 Antibody (S07-2K8)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1/50

Western Blot

1/1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

0.3 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SAP130

SAP130 encodes subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 3 has also been identified as a component of the STAGA (SPT3-TAF(II)31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF(II)-containing complex). These complexes may function in chromatin modification, transcription, splicing, and DNA repair.

Alternate Names

KIAA0017splicing factor 3b, subunit 3, 130kD, Pre-mRNA-splicing factor SF3b 130 kDa subunit, RSE1, SAP130SAP 130, SF3B130, SF3b130pre-mRNA splicing factor SF3b, 130 kDa subunit, Spliceosome-associated protein 130, splicing factor 3B subunit 3, splicing factor 3b, subunit 3, 130kDa, STAF130

Gene Symbol

SF3B3

Additional SAP130 Products

Product Documents for SAP130 Antibody (S07-2K8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SAP130 Antibody (S07-2K8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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