SAP130 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1077

Novus Biologicals, part of Bio-Techne
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NB100-1077
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

ELISA, Immunohistochemistry, Immunohistochemistry-Frozen, Peptide ELISA, Western Blot

Cited:

ELISA, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all SAP130 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-VSKKLEDIRTRYAF corresponding to C-Terminus according to NP_036558.3.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Bovine.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

136 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SAP130 Antibody

Western Blot: SAP130 Antibody [NB100-1077]

Western Blot: SAP130 Antibody [NB100-1077]

Western Blot: SAP130 Antibody [NB100-1077] - Staining (0.1ug/ml) of nuclear lysate from HeLa cells (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence

Applications for SAP130 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:2000

Western Blot

0.1 - 0.3 ug/ml
Application Notes
WB: Approx. 150 kDa band observed in HeLa nuclear lysates (calculated MW of 136 kDa band according to NP_036558.3). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 23594796). Use in ELISA reported in scientific literature (PMID: 22564223).

Reviewed Applications

Read 1 review rated 5 using NB100-1077 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SAP130

This gene encodes subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 3 has also been identified as a component of the STAGA (SPT3-TAF(II)31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF(II)-containing complex). These complexes may function in chromatin modification, transcription, splicing, and DNA repair.

Alternate Names

KIAA0017splicing factor 3b, subunit 3, 130kD, Pre-mRNA-splicing factor SF3b 130 kDa subunit, RSE1, SAP130SAP 130, SF3B130, SF3b130pre-mRNA splicing factor SF3b, 130 kDa subunit, Spliceosome-associated protein 130, splicing factor 3B subunit 3, splicing factor 3b, subunit 3, 130kDa, STAF130

Entrez Gene IDs

23450 (Human); 101943 (Mouse)

Gene Symbol

SF3B3

UniProt

Q15393 (Human)

Additional SAP130 Products

Product Documents for SAP130 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SAP130 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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