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SAP130 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20258

Novus Biologicals, part of Bio-Techne
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NBP2-20258

Key Product Details

Species Reactivity

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human SAP130. The exact sequence is proprietary.

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SAP130 Antibody

Western Blot: SAP130 Antibody [NBP2-20258]

Western Blot: SAP130 Antibody [NBP2-20258]

Western Blot: SAP130 Antibody [NBP2-20258] - Sample (30 ug of whole cell lysate) A: MCF-7 7. 5% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: SAP130 Antibody [NBP2-20258]

Immunocytochemistry/ Immunofluorescence: SAP130 Antibody [NBP2-20258]

Immunocytochemistry/Immunofluorescence: SAP130 Antibody [NBP2-20258] - Analysis of paraformaldehyde-fixed MCF-7, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: SAP130 Antibody [NBP2-20258]

Immunohistochemistry-Paraffin: SAP130 Antibody [NBP2-20258]

Immunohistochemistry-Paraffin: SAP130 Antibody [NBP2-20258] - Immunohistochemical analysis of paraffin-embedded PC14 xenograft, using antibody at 1:500 dilution.

Applications for SAP130 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SAP130

SAP130 is a subunit of the histone deacetylase (see HDAC1; MIM 601241)-dependent SIN3A (MIM 607776) corepressor complex (Fleischer et al., 2003 [PubMed 12724404]).[supplied by OMIM]

Alternate Names

KIAA0017splicing factor 3b, subunit 3, 130kD, Pre-mRNA-splicing factor SF3b 130 kDa subunit, RSE1, SAP130SAP 130, SF3B130, SF3b130pre-mRNA splicing factor SF3b, 130 kDa subunit, Spliceosome-associated protein 130, splicing factor 3B subunit 3, splicing factor 3b, subunit 3, 130kDa, STAF130

Gene Symbol

SF3B3

UniProt

Additional SAP130 Products

Product Documents for SAP130 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SAP130 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.