SAP155 Antibody (6C7)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26326
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Human, Mouse
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 6C7
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from Human SAP155 [UniProt O75533]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SAP155 Antibody (6C7)
Immunohistochemistry: SAP155 Antibody (6C7) [NBP3-26326] -
Immunohistochemistry: SAP155 Antibody (6C7) [NBP3-26326] - Image of SAP155 Antibody (6C7) diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunohistochemistry: SAP155 Antibody (6C7) [NBP3-26326] -
Immunohistochemistry: SAP155 Antibody (6C7) [NBP3-26326] - Image of SAP155 Antibody (6C7) diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Western Blot: SAP155 Antibody (6C7) [NBP3-26326] -
Western Blot: SAP155 Antibody (6C7) [NBP3-26326] - Positive Western Blot detected in: Hela whole cell lysate, K562 whole cell lysate, U-87 whole cell lysate, Mouse Brain whole cell lysate.All lanes: SAP155 Antibody at 1: 1000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 146, 17 kDa
Observed band size: 130 kDa
Applications for SAP155 Antibody (6C7)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:20-1:200
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:5000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: SAP155
Alternate Names
Hsh155, pre-mRNA processing 10, pre-mRNA splicing factor SF3b, 155 kDa subunit, Pre-mRNA-splicing factor SF3b 155 kDa subunit, Prp10, PRPF10, SAP 155, SAP155splicing factor 3b, subunit 1, 155kD, SF3B155, SF3b155PRP10, spliceosome associated protein 155, Spliceosome-associated protein 155, splicing factor 3B subunit 1, splicing factor 3b, subunit 1, 155kDa
Gene Symbol
SF3B1
Additional SAP155 Products
Product Documents for SAP155 Antibody (6C7)
Product Specific Notices for SAP155 Antibody (6C7)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...