SAP155 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-55255
Key Product Details
Validated by
Knockout/Knockdown, Independent Antibodies, Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human
Predicted:
Mouse (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunoprecipitation, Knockdown Validated, Simple Western, Western Blot
Cited:
Simple Western
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.2 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 100 and 150 of human splicing factor 3b, subunit 1, 155kDa using the numbering given in entry NP_036565.2 (GeneID 23451).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SAP155 Antibody
Knockdown Validated: SAP155 Antibody [NB100-55255]
Knockdown Validated: SAP155 Antibody [NB100-55255] - In vivo Smarce1-binding sites were detected by chromatin immunoprecipitation using a SMARCE1 antibody. Cropped gel images representing typical results from ChIP assays in: the regulatory module located from the transcription initiation site +1 to -1415, which includes the basic promoter (gata5-Bp), the module located from -1469 to -1892 (gata5-A), the module located from -7823 to -8213 (gata5-B) and the module located from -16805 to -17147 (gata5-C). IgG, Smarce1, Smarca4, Arid1a and Pbrm indicate the PCR products from genomic DNA precipitated using the corresponding antibody. WT and Mut indicate the source of genomic DNA. The input is the PCR product from the total input genomic DNA. Full-length gels are presented in Supplementary Figure 3. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41598-018-33746-8), licensed under a CC-BY license.Applications for SAP155 Antibody
Application
Recommended Usage
Immunoprecipitation
2-5 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Use in SW reported in secitific publication PMID: 32427851
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: SAP155
Alternate Names
Hsh155, pre-mRNA processing 10, pre-mRNA splicing factor SF3b, 155 kDa subunit, Pre-mRNA-splicing factor SF3b 155 kDa subunit, Prp10, PRPF10, SAP 155, SAP155splicing factor 3b, subunit 1, 155kD, SF3B155, SF3b155PRP10, spliceosome associated protein 155, Spliceosome-associated protein 155, splicing factor 3B subunit 1, splicing factor 3b, subunit 1, 155kDa
Entrez Gene IDs
23451 (Human)
Gene Symbol
SF3B1
UniProt
Additional SAP155 Products
Product Documents for SAP155 Antibody
Product Specific Notices for SAP155 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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