SAP155 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47291

Novus Biologicals, part of Bio-Techne
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NBP2-47291
NBP2-47291-25ul
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SAP155 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: LDSTGYYDQEIYGGSDSRFAGYVTSIAATELEDDDDDYSSSTSLLGQKKPGYHAPVALLNDIPQSTEQYDPFAEHRPPKIADRED

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SAP155 Antibody

Western Blot: SAP155 Antibody [NBP2-47291]

Western Blot: SAP155 Antibody [NBP2-47291]

Western Blot: SAP155 Antibody [NBP2-47291] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented.
Immunocytochemistry/ Immunofluorescence: SAP155 Antibody [NBP2-47291]

Immunocytochemistry/ Immunofluorescence: SAP155 Antibody [NBP2-47291]

Immunocytochemistry/Immunofluorescence: SAP155 Antibody [NBP2-47291] - Immunofluorescent staining of human cell line HeLa shows localization to nuclear speckles.
Immunohistochemistry-Paraffin: SAP155 Antibody [NBP2-47291]

Immunohistochemistry-Paraffin: SAP155 Antibody [NBP2-47291]

Immunohistochemistry-Paraffin: SAP155 Antibody [NBP2-47291] - Immunohistochemical staining of human salivary gland shows strong nuclear and cytoplasmic positivity in glandular cells.

Applications for SAP155 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SAP155

SF3B155 is a subunit of the U2 small nuclear ribonucleoproteins complex (U2 snRNP) that functions in pre-mRNA splicing. SF3B155 together with splicing factor 3a binds pre-mRNA upstream of the branchpoint sequence to anchor U2 snRNP to the pre-mRNA. SF3B155 is also a component of the U12-type splicesome that is involved in the splicing of a rare class of nuclear pre-mRNA introns.

Alternate Names

Hsh155, pre-mRNA processing 10, pre-mRNA splicing factor SF3b, 155 kDa subunit, Pre-mRNA-splicing factor SF3b 155 kDa subunit, Prp10, PRPF10, SAP 155, SAP155splicing factor 3b, subunit 1, 155kD, SF3B155, SF3b155PRP10, spliceosome associated protein 155, Spliceosome-associated protein 155, splicing factor 3B subunit 1, splicing factor 3b, subunit 1, 155kDa

Gene Symbol

SF3B1

Additional SAP155 Products

Product Documents for SAP155 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SAP155 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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