SAP18 Antibody (3B2)

Novus Biologicals, part of Bio-Techne | Catalog # H00010284-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3B2

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SAP18 Antibodies »

Product Specifications

Immunogen

SAP18 (AAH30836, 1 a.a. ~ 153 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAVESRVTQEEIKKEPEKPIDREKTCPLLLRVFTTNNGRHHRMDEFSRGNVPSSELQIYTWMDATLKELTSLVKEVYPEARKKGTHFNFAIVFTDVKRPGYRVKEIGSTMSGRKGTDDSMTLQSQKFQIGDYLDIAITPPNRAPPTSGRMRPY

Reactivity Notes

Human. Other species not tested.

Specificity

SAP18 (3B2)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for SAP18 Antibody (3B2)

Immunocytochemistry/ Immunofluorescence: SAP18 Antibody (3B2) [H00010284-M01]

Immunocytochemistry/ Immunofluorescence: SAP18 Antibody (3B2) [H00010284-M01]

Immunocytochemistry/Immunofluorescence: SAP18 Antibody (3B2) [H00010284-M01] - Analysis of monoclonal antibody to SAP18 on HeLa cell . Antibody concentration 10 ug/ml.
Sandwich ELISA: SAP18 Antibody (3B2) [H00010284-M01] - Detection limit for recombinant GST tagged SAP18 is approximately 0.1ng/ml as a capture antibody.

Applications for SAP18 Antibody (3B2)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody Reactive against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SAP18

Histone acetylation plays a key role in the regulation of eukaryotic gene expression. Histone acetylation and deacetylation are catalyzed by multisubunit complexes. The protein encoded by this gene is a component of the histone deacetylase complex, which includes SIN3, SAP30, HDAC1, HDAC2, RbAp46, RbAp48, and other polypeptides. This protein directly interacts with SIN3 and enhances SIN3-mediated transcriptional repression when tethered to the promoter.

Alternate Names

2HOR0202cell growth inhibiting protein 38, Cell growth-inhibiting gene 38 protein, cell growth-inhibiting protein 38, histone deacetlyase complex subunit SAP18, histone deacetylase complex subunit SAP18,18 kDa Sin3-associated polypeptide, MGC27131, SAP18P, Sin3A-associated protein, 18kDa, Sin3-associated polypeptide p18, sin3-associated polypeptide, 18 kDa, sin3-associated polypeptide, p18

Entrez Gene IDs

10284 (Human)

Gene Symbol

SAP18

OMIM

602949 (Human)

UniProt

AAH30836 (Human)

Additional SAP18 Products

Product Documents for SAP18 Antibody (3B2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SAP18 Antibody (3B2)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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