SAP30 Antibody (4D2)

Novus Biologicals, part of Bio-Techne | Catalog # H00008819-M02

Submit a review

Product Datasheet / COA / SDS

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 4D2

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SAP30 Antibodies »

Product Specifications

Immunogen

SAP30 (NP_003855, 131 a.a. ~ 219 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SDDDGGDSPVQDIDTPEVDLYQLQVNTLRRYKRHFKLPTRPGLNKAQLVEIVGCHFRSIPVNEKDTLTYFIYSVKNDKNKSDLKVDSGV

Specificity

SAP30 (4D2)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for SAP30 Antibody (4D2)

Immunocytochemistry/ Immunofluorescence: SAP30 Antibody (4D2) [H00008819-M02]

Immunocytochemistry/Immunofluorescence: SAP30 Antibody (4D2) [H00008819-M02] - Analysis of monoclonal antibody to SAP30 on HeLa cell . Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: SAP30 Antibody (4D2) [H00008819-M02]

Immunohistochemistry-Paraffin: SAP30 Antibody (4D2) [H00008819-M02] - Analysis of monoclonal antibody to SAP30 on formalin-fixed paraffin-embedded human esophagus. Antibody concentration 1.5 ug/ml.

ELISA: SAP30 Antibody (4D2) [H00008819-M02]

ELISA: SAP30 Antibody (4D2) [H00008819-M02] - Detection limit for recombinant GST tagged SAP30 is approximately 1ng/ml as a capture antibody.

Applications for SAP30 Antibody (4D2)

Application

Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Antibody reactivity against recombinant protein for WB. It has been used for IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SAP30

Histone acetylation plays a key role in the regulation of eukaryotic gene expression. Histone acetylation and deacetylation are catalyzed by multisubunit complexes. The protein encoded by this gene is a component of the histone deacetylase complex, which includes SIN3, SAP18, HDAC1, HDAC2, RbAp46, RbAp48, and other polypeptides. This complex is active in deacetylating core histone octamers, but inactive in deacetylating nucleosomal histones. A pseudogene of this gene is located on chromosome 3.

Alternate Names

30 kDa Sin3-associated polypeptide, histone deacetylase complex subunit SAP30, Sin3 corepressor complex subunit SAP30, Sin3A-associated protein, 30kDa, Sin3-associated polypeptide p30, sin3-associated polypeptide, 30 kDa, Sin3-associated polypeptide, 30kDa

Entrez Gene IDs

8819 (Human)

Gene Symbol

SAP30

OMIM

603378 (Human)

UniProt

NP_003855 (Human)

Additional SAP30 Products

Product Documents for SAP30 Antibody (4D2)

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for SAP30 Antibody (4D2)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews