Key Product Details

Species Reactivity

Validated:

SARS-CoV-2

Predicted:

SARS-CoV (100%). Backed by our 100% Guarantee.

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all 3CL Protease Antibodies »

Product Specifications

Immunogen

This antibody was raised against a peptide corresponding to 16 amino acids near the amino terminus of SARS-CoV-2 (COVID-19) 3CL-Pro (NSP5) protein. The immunogen is located within the first 50 amino acids of the SARS-CoV-2 (COVID-19) 3CL-Pro (NSP5) protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SARS-CoV-2 3CL Protease Antibody - BSA Free

Western Blot: SARS-CoV-2 3CL Protease AntibodyBSA Free [NBP3-07062]

Western Blot: SARS-CoV-2 3CL Protease AntibodyBSA Free [NBP3-07062]

Western Blot: SARS-CoV-2 3CL Protease Antibody [NBP3-07062] - Western Blot Validation with SARS-CoV-2 3CL Protease Protein. Loading: 30 ng per lane of SARS-CoV-2 3CL Protease recombinant protein. Antibodies: SARS-CoV-2 3CL Protease, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.5 ug/ml and Lane 2: 1 ug/ml
Immunohistochemistry-Paraffin: SARS-CoV-2 3CL Protease Antibody - BSA Free [NBP3-07062]

Immunohistochemistry-Paraffin: SARS-CoV-2 3CL Protease Antibody - BSA Free [NBP3-07062]

Immunohistochemistry-Paraffin: SARS-CoV-2 3CL Protease Antibody [NBP3-07062] - Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 3CL protease antibody (0.5 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 3L-PRO (NSP5) protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.
ELISA: SARS-CoV-2 3CL Protease Antibody - BSA Free [NBP3-07062]

ELISA: SARS-CoV-2 3CL Protease Antibody - BSA Free [NBP3-07062]

ELISA: SARS-CoV-2 3CL Protease Antibody [NBP3-07062] - Antibodies: SARS-CoV-2 3CL Protease antibody. A direct ELISA was performed using SARS-CoV-2 3CL Protease recombinant protein as coating antigen and SARS-CoV-2 3CL Protease antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 3000 ng/mL

Applications for SARS-CoV-2 3CL Protease Antibody - BSA Free

Application
Recommended Usage

ELISA

1 ug/mL

Immunohistochemistry

0.5 ug/mL

Immunohistochemistry-Paraffin

0.5 ug/mL

Western Blot

0.5 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: 3CL Protease

Long Name

3CL Protease

Alternate Names

Main Protease, Mpro

Gene Symbol

ORF1ab

Additional 3CL Protease Products

Product Documents for SARS-CoV-2 3CL Protease Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SARS-CoV-2 3CL Protease Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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