SARS-CoV-2 Membrane Protein Antibody (HL1088) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25648

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-25648
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Key Product Details

Species Reactivity

SARS-CoV-2

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1088

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SARS-CoV-2 Membrane Protein Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of SARS-CoV-2 Membrane Protein. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SARS-CoV-2 Membrane Protein Antibody (HL1088) - Azide and BSA Free

SARS-CoV-2 Membrane Protein Antibody (HL1088) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: SARS-CoV-2 Membrane Protein Antibody (HL1088) - Azide and BSA Free [NBP3-25648] -

Immunocytochemistry/Immunofluorescence: SARS-CoV-2 Membrane Protein Antibody (HL1088) - Azide and BSA Free [NBP3-25648] - SARS-CoV-2 (COVID-19) Membrane antibody [HL1088] detects SARS-CoV-2 (COVID-19) Membrane protein by immunofluorescent analysis. Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SARS-CoV-2 (COVID-19) Membrane stained by SARS-CoV-2 (COVID-19) Membrane antibody [HL1088] (NBP3-25648) diluted at 1:2000. Blue: Fluoroshield with DAPI .
SARS-CoV-2 Membrane Protein Antibody (HL1088) - Azide and BSA Free

Western Blot: SARS-CoV-2 Membrane Protein Antibody (HL1088) - Azide and BSA Free [NBP3-25648] -

Western Blot: SARS-CoV-2 Membrane Protein Antibody (HL1088) - Azide and BSA Free [NBP3-25648] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Membrane antibody [HL1088] (NBP3-25648) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for SARS-CoV-2 Membrane Protein Antibody (HL1088) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SARS-CoV-2 Membrane Protein

The SARS-CoV-2 Membrane protein is one of the four major structural proteins of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the causative agent of COVID-19 (1). The membrane protein is the most abundant of the four structural proteins, which also includes the envelope protein, spike protein, and nucleocapsid protein (1,2). The membrane protein is synthesized as a 222 amino acid protein with a theoretical molecular weight of 25.1 kDa (2,3). Furthermore, the membrane protein of SARS-CoV-2 has 90.5% sequence identity and 98.2% sequence similarity to that of the membrane protein of SARS-CoV (2). Structurally, the membrane protein is a type III transmembrane glycoprotein containing a glycosylated N-terminal ectodomain, three transmembrane domains, and a large C-terminal that extends into the viral particle (2,4). The membrane protein exists as a dimer and is either in long or compact conformation, which contributes to the curvature of the viral membrane (2,4). The main functions of the membrane protein are for viral assembly and contributing to the shape and structural integrity of the viral envelope (1,2). Additional functions show a role in inducing apoptosis as well as interactions with the spike and nucleocapsid proteins (1,2). The membrane-spike protein interaction is required for maintaining the spike protein within the ER-Golgi intermediate compartment (1). The membrane protein binding with the nucleocapsid protein is responsible for nucleocapsid stabilization and viral assembly (1).

References

1. Malik Y. A. (2020). Properties of Coronavirus and SARS-CoV-2. The Malaysian Journal of Pathology.

2. Yoshimoto F. K. (2020). The Proteins of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS CoV-2 or n-COV19), the Cause of COVID-19. The Protein Journal. https://doi.org/10.1007/s10930-020-09901-4

3. Uniprot (P0DTC5)

4. J Alsaadi, E. A., & Jones, I. M. (2019). Membrane binding proteins of coronaviruses. Future Virology. https://doi.org/10.2217/fvl-2018-0144

Alternate Names

2019-nCoV M protein, 2019-nCoV Membrane Protein, COVID-19 E1 Glycoprotein, COVID-19 Matrix glycoprotein, COVID-19 Membrane, COVID-19 Membrane glycoprotein, E1 glycoprotein, Human coronavirus Membrane Protein, Matrix glycoprotein, Membrane glycoprotein, Membrane Protein, SARS-CoV-2, SARS-CoV-2 E1 Glycoprotein, SARS-CoV-2 M Protein, SARS-CoV-2 Matrix glycoprotein, SARS-CoV-2 Membrane glycoprotein, SARSCoV2 Membrane Protein, Severe Acute Respiratory Syndrome Coronavirus 2

Gene Symbol

M

Additional SARS-CoV-2 Membrane Protein Products

Product Documents for SARS-CoV-2 Membrane Protein Antibody (HL1088) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SARS-CoV-2 Membrane Protein Antibody (HL1088) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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