SARS-CoV-2 nsp12 Antibody (HL1444) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25653
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
SARS-CoV-2
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL1444
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of SARS-CoV-2 nsp12 / RdRP. The exact sequence is proprietary.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SARS-CoV-2 nsp12 Antibody (HL1444) - Azide and BSA Free
Immunocytochemistry/Immunofluorescence: SARS-CoV-2 nsp12 Antibody (HL1444) - Azide and BSA Free [NBP3-25653] -
Immunocytochemistry/Immunofluorescence: SARS-CoV-2 nsp12 Antibody (HL1444) - Azide and BSA Free [NBP3-25653] - SARS-CoV-2 (COVID-19) nsp12 / RdRP antibody [HL1444] detects SARS-CoV-2 (COVID-19) nsp12 / RdRP protein at cytoplasm by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SARS-CoV-2 (COVID-19) nsp12 / RdRP stained by SARS-CoV-2 (COVID-19) nsp12 / RdRP antibody [HL1444] (NBP3-25653) diluted at 1:500. Blue: Fluoroshield with DAPI . Scale bar= 10um.
Immunohistochemistry-Paraffin: SARS-CoV-2 nsp12 Antibody (HL1444) - Azide and BSA Free [NBP3-25653] -
Immunohistochemistry-Paraffin: SARS-CoV-2 nsp12 Antibody (HL1444) - Azide and BSA Free [NBP3-25653] - SARS-CoV-2 nsp12 detects SARS-CoV-2 nsp12 protein at cytoplasm by immunohistochemical analysis. Sample: human SARS-CoV-2 (COVID-19) Nucleocapsid transfected 293T cell FFPE Cell Pellet Block . Green: SARS-CoV-2 nsp12 stained by SARS-CoV-2 nsp12 (NBP3-25653) diluted at 1:255. Blue: Fluoroshield with DAPI . Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: SARS-CoV-2 nsp12 Antibody (HL1444) - Azide and BSA Free [NBP3-25653] -
Western Blot: SARS-CoV-2 nsp12 Antibody (HL1444) - Azide and BSA Free [NBP3-25653] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nsp12 / RdRP antibody [HL1444] (NBP3-25653) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Applications for SARS-CoV-2 nsp12 Antibody (HL1444) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Assay dependent
Immunohistochemistry
Assay dependent
Immunohistochemistry-Paraffin
Assay dependent
Western Blot
1:1000-1:10000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SARS-CoV-2 nsp12
Alternate Names
Non-structural protein 12, nsp12, ORF1a polyprotein, ORF1ab polyprotein, pp1a, Replicase polyprotein 1a
Gene Symbol
ORF1ab
Additional SARS-CoV-2 nsp12 Products
Product Documents for SARS-CoV-2 nsp12 Antibody (HL1444) - Azide and BSA Free
Product Specific Notices for SARS-CoV-2 nsp12 Antibody (HL1444) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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