Key Product Details

Species Reactivity

Validated:

SARS-CoV-2

Predicted:

SARS-CoV (100%). Backed by our 100% Guarantee.

Applications

ELISA, Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all SARS-CoV-2 nsp7 Antibodies »

Product Specifications

Immunogen

This antibody was raised against a peptide corresponding to 12 amino acids near the carboxyl terminus of the SARS-CoV-2 (COVID-19) NSP7 protein. The immunogen is located within the last 50 amino acids of the SARS-CoV-2 (COVID-19) NSP7 protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SARS-CoV-2 nsp7 Antibody - BSA Free

Immunohistochemistry: SARS-CoV-2 nsp7 Antibody - BSA Free [NBP3-07048]

Immunohistochemistry: SARS-CoV-2 nsp7 Antibody - BSA Free [NBP3-07048]

Immunohistochemistry: SARS-CoV-2 nsp7 Antibody [NBP3-07048] - Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) NSP7 anbody (0.5 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 degrees celsius. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 NSP7 protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.
ELISA: SARS-CoV-2 nsp7 Antibody - BSA Free [NBP3-07048]

ELISA: SARS-CoV-2 nsp7 Antibody - BSA Free [NBP3-07048]

ELISA: SARS-CoV-2 nsp7 Antibody [NBP3-07048] - Antibodies: SARS-CoV-2 (COVID-19) NSP7 Antibody. A direct ELISA was performed using SARS-CoV-2 NSP7 recombinant protein as coating antigen and the anti-SARS-CoV-2 (COVID-19) NSP7 antibody as the capture antibody. Secondary: Goat an-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 1000 ng/mL.

Applications for SARS-CoV-2 nsp7 Antibody - BSA Free

Application
Recommended Usage

ELISA

1 ug/mL

Immunohistochemistry

0.5 ug/mL

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SARS-CoV-2 nsp7

SARS-CoV-2 Nonstructural Protein 7 (NSP7) is one of the sixteen nonstructural proteins of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the causative agent of COVID-19 (1). SARS-CoV-2 NSP7 is 83 amino acids (aa) with a theoretical molecular weight of 9.2 kDa (1,2). The amino acid sequence alignment of SARS-CoV and SARS-CoV-2's NSP7 has 98.8% sequence identity and 100% sequence similarity (1). Functionally, SARS-Cov-2 NSP7 forms a complex with NSP8 and NSP12 to generate NSP8's RNA polymerase activity (1-3). In SARS-CoV, NSP7 and NSP8 are required for viral survival and form a channel-like, cylinder supercomplex (3). The NSP7-NSP8-NSP12 complex is considered the minimal core complex for RNA synthesis (4). While structurally similar to SARS-CoV, the components of the NSP7-NSP8-NSP12 complex of SARS-CoV-2 have reduced polymerase activity and thermostability (4).

References

1. Yoshimoto F. K. (2020). The Proteins of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS CoV-2 or n-COV19), the Cause of COVID-19. The Protein Journal. https://doi.org/10.1007/s10930-020-09901-4

2. Gordon, D. E., Jang, G. M., Bouhaddou, M., Xu, J., Obernier, K., White, K. M., O'Meara, M. J., Rezelj, V. V., Guo, J. Z., Swaney, D. L., Tummino, T. A., Huttenhain, R., Kaake, R. M., Richards, A. L., Tutuncuoglu, B., Foussard, H., Batra, J., Haas, K., Modak, M., Kim, M., ... Krogan, N. J. (2020). A SARS-CoV-2 protein interaction map reveals targets for drug repurposing. Nature. https://doi.org/10.1038/s41586-020-2286-9

3. Qiu, Y., & Xu, K. (2020). Functional studies of the coronavirus nonstructural proteins. STEMedicine. https://doi.org/10.37175/stemedicine.v1i2.39

4. Peng, Q., Peng, R., Yuan, B., Zhao, J., Wang, M., Wang, X., Wang, Q., Sun, Y., Fan, Z., Qi, J., Gao, G. F., & Shi, Y. (2020). Structural and Biochemical Characterization of the nsp12-nsp7-nsp8 Core Polymerase Complex from SARS-CoV-2. Cell reports. https://doi.org/10.1016/j.celrep.2020.107774

Alternate Names

Non-structural protein 7, nsp7 replicase

Gene Symbol

ORF1ab

Additional SARS-CoV-2 nsp7 Products

Product Documents for SARS-CoV-2 nsp7 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SARS-CoV-2 nsp7 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.