SARS-CoV-2 nsp7 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13470

Novus Biologicals, part of Bio-Techne
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NBP3-13470
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Key Product Details

Species Reactivity

SARS-CoV-2

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SARS-CoV-2 nsp7 Antibodies »

Product Specifications

Immunogen

Full length SARS-CoV-2 nsp7 recombinant protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for SARS-CoV-2 nsp7 Antibody

Western Blot: SARS-CoV-2 nsp7 Antibody [NBP3-13470]

Western Blot: SARS-CoV-2 nsp7 Antibody [NBP3-13470]

Western Blot: SARS-CoV-2 nsp7 Antibody [NBP3-13470] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nsp7 antibody (NBP3-13470) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Applications for SARS-CoV-2 nsp7 Antibody

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SARS-CoV-2 nsp7

SARS-CoV-2 Nonstructural Protein 7 (NSP7) is one of the sixteen nonstructural proteins of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the causative agent of COVID-19 (1). SARS-CoV-2 NSP7 is 83 amino acids (aa) with a theoretical molecular weight of 9.2 kDa (1,2). The amino acid sequence alignment of SARS-CoV and SARS-CoV-2's NSP7 has 98.8% sequence identity and 100% sequence similarity (1). Functionally, SARS-Cov-2 NSP7 forms a complex with NSP8 and NSP12 to generate NSP8's RNA polymerase activity (1-3). In SARS-CoV, NSP7 and NSP8 are required for viral survival and form a channel-like, cylinder supercomplex (3). The NSP7-NSP8-NSP12 complex is considered the minimal core complex for RNA synthesis (4). While structurally similar to SARS-CoV, the components of the NSP7-NSP8-NSP12 complex of SARS-CoV-2 have reduced polymerase activity and thermostability (4).

References

1. Yoshimoto F. K. (2020). The Proteins of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS CoV-2 or n-COV19), the Cause of COVID-19. The Protein Journal. https://doi.org/10.1007/s10930-020-09901-4

2. Gordon, D. E., Jang, G. M., Bouhaddou, M., Xu, J., Obernier, K., White, K. M., O'Meara, M. J., Rezelj, V. V., Guo, J. Z., Swaney, D. L., Tummino, T. A., Huttenhain, R., Kaake, R. M., Richards, A. L., Tutuncuoglu, B., Foussard, H., Batra, J., Haas, K., Modak, M., Kim, M., ... Krogan, N. J. (2020). A SARS-CoV-2 protein interaction map reveals targets for drug repurposing. Nature. https://doi.org/10.1038/s41586-020-2286-9

3. Qiu, Y., & Xu, K. (2020). Functional studies of the coronavirus nonstructural proteins. STEMedicine. https://doi.org/10.37175/stemedicine.v1i2.39

4. Peng, Q., Peng, R., Yuan, B., Zhao, J., Wang, M., Wang, X., Wang, Q., Sun, Y., Fan, Z., Qi, J., Gao, G. F., & Shi, Y. (2020). Structural and Biochemical Characterization of the nsp12-nsp7-nsp8 Core Polymerase Complex from SARS-CoV-2. Cell reports. https://doi.org/10.1016/j.celrep.2020.107774

Alternate Names

Non-structural protein 7, nsp7 replicase

Gene Symbol

ORF1ab

Additional SARS-CoV-2 nsp7 Products

Product Documents for SARS-CoV-2 nsp7 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SARS-CoV-2 nsp7 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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