SARS-CoV-2 Nucleocapsid Antibody (004) [Allophycocyanin]

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12734APC

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

SARS-CoV

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Rabbit IgG Clone # 004

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SARS-CoV-2 Nucleocapsid Antibodies »

Product Specifications

Immunogen

Recombinant SARS-CoV-2 Nucleocapsid Protein

Reactivity Notes

Has cross-reactivity in ELISA and WB with:
SARS-CoV-2 (2019-nCoV) Nucleocapsid Protein

No cross-reactivity in ELISA with:
MERS-CoV Nucleoprotein protein
HCoV-229E Nucleoprotein protein
HCoV-NL63 Nucleoprotein protein
HCoV-HKU1(isolate N5) Nucleoprotein protein
HCoV-OC43 Nucleoprotein

Specificity

Has cross-reactivity in ELISA and WB with:SARS-CoV-2 Nucleocapsid ProteinHas cross-reactivity in ELISA with:SARS-CoV-2 Omicron (B.1.1.529) Nucleocapsid ProteinSARS-CoV-2 Nucleocapsid ProteinSARS-CoV-2 Alpha(B.1.1.7) Nucleocapsid(R203K, G204R) ProteinSARS-CoV-2 Alpha(B.1.1.7) Nucleocapsid(I292T) ProteinSARS-CoV-2 Alpha(B.1.1.7) Nucleocapsid(D3L, R203K, G204R, S235F) ProteinSARS-CoV-2 Alpha(B.1.1.7) Nucleocapsid(D3L, S235F) ProteinSARS-CoV-2 Alpha(B.1.1.7/ B.1.237) Nucleocapsid(S194L) ProteinSARS-CoV-2 Alpha/beta(B.1.1.7/ B.1.351/ A.2.2) Nucleocapsid(P13L) ProteinSARS-CoV-2 Beta(B.1.351/B.1.351.2/b.1.351.3/B.1.427/B.1.429) Nucleocapsid (T205I) ProteinSARS-CoV-2 Gamma(P.1/ P.1.1/ P.1.2) Nucleocapsid (P80R) ProteinSARS-CoV-2 Eta(B.1.525) Nucleocapsid (A12G, T205I) ProteinSARS-CoV-2 (B.1.617) Nucleocapsid (D377Y) ProteinSARS-CoV-2 (B.1.617) Nucleocapsid (R203M, D377Y) ProteinSARS-CoV-2 Delta(B.1.617.2) Nucleocapsid (D63G, R203M, D377Y) ProteinSARS-CoV-2 (B.1.617.3) Nucleocapsid (P67S, R203M, D377Y) ProteinNo cross-reactivity in ELISA with:MERS-CoV Nucleocapsid proteinHCoV-229E Nucleocapsid proteinHCoV-NL63 Nucleocapsid proteinHCoV-HKU1(isolate N5) Nucleocapsid proteinHCoV-OC43 Nucleocapsid protein

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Applications for SARS-CoV-2 Nucleocapsid Antibody (004) [Allophycocyanin]

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: SARS-CoV-2 Nucleocapsid

The SARS-CoV-2 Nucleocapsid protein is one of the four major structural proteins of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the causative agent of COVID-19 (1,2). The nucleocapsid protein is an RNA-binding protein that is essential for viral assembly into a ribonucleoprotein complex and also functions in viral budding (2,3). More specifically, after viral replication and RNA synthesis, the viral genomes that are encapsulated by the nucleocapsid protein will bud into the membrane and help with mature virus formation (2). The nucleocapsid protein is synthesized as 419 amino acids (aa) in length with a theoretical molecular weight of 45.6 kDa (4). Structurally, the nucleocapsid protein contains an RNA-binding domain (N1b), a dimerization domain (N2b), and a few shorter regions (N1a, N2a, and spacer B/N3) (3). It is the N1b domain that is suggested to be involved in RNA binding (3). The nucleocapsid N1b domain contains a beta-hairpin, a loop region, and a beta-sheet core, giving it a right-handed shape (3). Conversely, the N2b domain functions in dimerization which promotes assembly into a helical filament where the dimer interface is made of two beta-strands and an alpha-helix portion (3). Inhibition of nucleocapsid assembly may be a potential target for treating COVID-19 infection.

Following SARS-CoV-2 infection, B cells and T cells display an immune response against nucleocapsid protein and nucleocapsid-specific neutralizing antibodies are produced (5). Interestingly, a study of patients who have recovered from COVID-19 revealed that there were no neutralizing antibodies against nucleocapsid protein present; however, a high presence against neutralizing spike RBD antibodies were detected (5).

References

1. Pillay T. S. (2020). Gene of the month: the 2019-nCoV/SARS-CoV-2 novel coronavirus spike protein. Journal of Clinical Pathology. https://doi.org/10.1136/jclinpath-2020-206658

2. Malik Y. A. (2020). Properties of Coronavirus and SARS-CoV-2. The Malaysian Journal of Pathology.

3. Zhu, G., Zhu, C., Zhu, Y., & Sun, F. (2020). Minireview of progress in the structural study of SARS-CoV-2 proteins. Current Research in Microbial Sciences. https://doi.org/10.1016/j.crmicr.2020.06.003

4. Uniprot (P0DTC9)

5. Shah, V. K., Firmal, P., Alam, A., Ganguly, D., & Chattopadhyay, S. (2020). Overview of Immune Response During SARS-CoV-2 Infection: Lessons From the Past. Frontiers in Immunology. https://doi.org/10.3389/fimmu.2020.01949

Alternate Names

2019-nCoV N Protein, 2019-nCoV Nucleocapsid Protein, COVID-19 N protein, COVID-19 nucleocapsid protein, Human coronavirus Nucleoprotein, nucleocapsid phosphoprotein, Nucleocapsid protein, Nucleoprotein, ORF9; structural protein, SARS-CoV-2, SARS-CoV-2 Nucleocapsid protein, SARS-CoV-2 N protein, SARSCoV2 Nucleoprotein, Severe Acute Respiratory Syndrome Coronavirus 2 Nucleocapsid Protein

Gene Symbol

N

Additional SARS-CoV-2 Nucleocapsid Products

Product Documents for SARS-CoV-2 Nucleocapsid Antibody (004) [Allophycocyanin]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SARS-CoV-2 Nucleocapsid Antibody (004) [Allophycocyanin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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