SARS-CoV-2 Spike S2 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05701

Novus Biologicals, part of Bio-Techne
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NBP3-05701
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Key Product Details

Species Reactivity

Validated:

SARS-CoV-2

Predicted:

SARS-CoV (100%). Backed by our 100% Guarantee.

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all Spike S2 Subunit Antibodies »

Product Specifications

Immunogen

SARS-CoV-2 Spike S2 antibody was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of SARS-CoV-2 Spike S2 glycoprotein. The immunogen is located within 1130-1180 amino acids of SARS-CoV-2 Spike S2 protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SARS-CoV-2 Spike S2 Antibody - BSA Free

Western Blot: SARS-CoV-2 Spike S2 AntibodyBSA Free [NBP3-05701]

Western Blot: SARS-CoV-2 Spike S2 AntibodyBSA Free [NBP3-05701]

Western Blot: SARS-CoV-2 Spike S2 Antibody [NBP3-05701] - Overexpression Validation in Spike Transfected 293 Cells. Loading: 10 ug per lane of Spike transfected 293 cell lysate. Antibodies: SARS-CoV-2 Spike S2, NBP3-05701, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 ug/mL and Lane 2: 2 ug/mL
Immunohistochemistry-Paraffin: SARS-CoV-2 Spike S2 Antibody - BSA Free [NBP3-05701]

Immunohistochemistry-Paraffin: SARS-CoV-2 Spike S2 Antibody - BSA Free [NBP3-05701]

Immunohistochemistry-Paraffin: SARS-CoV-2 Spike S2 Antibody [NBP3-05701] - Immunohistochemistry Validation of SARS-CoV-2 Spike S2 in COVID-19 Patient Lung. Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 Spike S2 antibody (NBP3-05701, 0.1 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 spike protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.
Western Blot: SARS-CoV-2 Spike S2 AntibodyBSA Free [NBP3-05701]

Western Blot: SARS-CoV-2 Spike S2 AntibodyBSA Free [NBP3-05701]

Western Blot: SARS-CoV-2 Spike S2 Antibody [NBP3-05701] - Western Blot Validation with SARS-CoV-2 Spike S2 ECD Recombinant Protein. Loading: 30 ng per lane of SARS-CoV-2 Spike S2 ECD recombinant protein. Antibodies: SARS-CoV-2 Spike S2, NBP3-05701, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 ug/mL and Lane 2: 2 ug/mL.
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Applications for SARS-CoV-2 Spike S2 Antibody - BSA Free

Application
Recommended Usage

ELISA

1 ug/mL

Immunocytochemistry/ Immunofluorescence

10ug/ml

Immunohistochemistry

0.1ug/ml

Western Blot

1 ug/mL

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Spike S2 Subunit

The SARS-CoV-2 Spike protein is one of the four major structural proteins of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the causative agent of COVID-19 (1,2). The spike protein is the largest of the structural proteins, which also include the membrane (M), envelope (E), and nucleocapsid (N) proteins (1,2). The SARS-CoV-2 spike protein is a 1273 amino acid (aa) heterotrimeric class I fusion protein with each monomer having a theoretical molecular weight of approximately 180 kDa (1). The club-shaped spike protein contains several functional regions and domains including the S1 globular head region which contains the N-terminal receptor-binding domain (RBD) and the S2 stem region that contains the C-terminal fusion domain, two heptad regions, a transmembrane domain, and a cytoplasmic tail (1,2). The viral spike protein is critical for attachment of the virus with the host cell, resulting in fusion and virus entry into the cell (1,2). More specifically, the RBD of the spike protein is responsible for binding to the cell surface receptor angiotensin converting enzyme 2 (ACE2) (1,2). This spike-ACE2 interaction results in a conformational change permitting furin cleavage between the S1 and S2 domains and then cleavage at S2' by TMPRRS2, or another protease, allowing membrane fusion (1,2).

Given the critical role of the spike protein RBD in the interaction with the ACE2 receptor and viral entry, a number of neutralizing antibodies against the RBD have been developed as potential therapeutics for treating COVID-19 (3). These antibodies bind the RBD domain on the S1 subunit inhibiting the interaction with ACE2 (3). However, more studies need to be done as neutralizing antibodies can result in antibody-dependent enhancement, in which the viral entry and replication within the host cell is increased (4). One potential way to combat antibody-dependent enhancement is the use of nanobodies (4). Furthermore, there are currently several vaccine strategies that are in clinical trials, or recently federally approved, that utilize the spike protein in different forms (e.g. full length, S1 RBD, RBD-Fc, N-terminal) for protecting against SARS-CoV-2 infection (4,5). These vaccine strategies include DNA vaccines, viral vector-based vaccines, RNA vaccines, and subunit vaccines (4,5).

References

1. Pillay T. S. (2020). Gene of the month: the 2019-nCoV/SARS-CoV-2 novel coronavirus spike protein. Journal of Clinical Pathology. https://doi.org/10.1136/jclinpath-2020-206658

2. Malik Y. A. (2020). Properties of Coronavirus and SARS-CoV-2. The Malaysian Journal of Pathology.

3. Ho M. (2020). Perspectives on the development of neutralizing antibodies against SARS-CoV-2. Antibody Therapeutics. https://doi.org/10.1093/abt/tbaa009

4. Samrat, S. K., Tharappel, A. M., Li, Z., & Li, H. (2020). Prospect of SARS-CoV-2 spike protein: Potential role in vaccine and therapeutic development. Virus Research. https://doi.org/10.1016/j.virusres.2020.198141

5. Sternberg, A., & Naujokat, C. (2020). Structural features of coronavirus SARS-CoV-2 spike protein: Targets for vaccination. Life Sciences. https://doi.org/10.1016/j.lfs.2020.118056

Long Name

Spike Protein, S2 Subunit

Alternate Names

COVID-19, S2 protein, SARS-CoV-2, SARS-CoV-2 Spike, Spike glycoprotein Subunit 2, spike protein, Spike protein S2, structural protein, surface glycoprotein

Gene Symbol

S

UniProt

QHD43416

Additional Spike S2 Subunit Products

Product Documents for SARS-CoV-2 Spike S2 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SARS-CoV-2 Spike S2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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