SARS Nucleocapsid Protein Antibody (001)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-90988
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Key Product Details
Species Reactivity
SARS-CoV, SARS-CoV-2
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 001
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant SARS-CoV Nucleoprotein/NP Protein
Reactivity Notes
This antibody has cross-reactivity in ELISA and WB with:
SARS-CoV-2 Nucleocapsid Protein
No cross-reactivity in ELISA with:
MERS-CoV Nucleoprotein protein
HCoV-229E Nucleoprotein protein
HCoV-NL63 Nucleoprotein protein
HCoV-HKU1(isolate N5) Nucleoprotein protein
HCoV-OC43 Nucleoprotein
SARS-CoV-2 Nucleocapsid Protein
No cross-reactivity in ELISA with:
MERS-CoV Nucleoprotein protein
HCoV-229E Nucleoprotein protein
HCoV-NL63 Nucleoprotein protein
HCoV-HKU1(isolate N5) Nucleoprotein protein
HCoV-OC43 Nucleoprotein
Specificity
Has cross-reactivity in ELISA and WB with:SARS-CoV-2 Nucleocapsid ProteinHas cross-reactivity in ELISA with:SARS-CoV-2 Omicron (B.1.1.529) Nucleocapsid ProteinSARS-CoV-2 Nucleocapsid ProteinSARS-CoV-2 Alpha(B.1.1.7) Nucleocapsid(R203K, G204R) ProteinSARS-CoV-2 Alpha(B.1.1.7) Nucleocapsid(I292T) ProteinSARS-CoV-2 Alpha(B.1.1.7) Nucleocapsid(D3L, R203K, G204R, S235F) ProteinSARS-CoV-2 Alpha(B.1.1.7) Nucleocapsid(D3L, S235F) ProteinSARS-CoV-2 Alpha(B.1.1.7/ B.1.237) Nucleocapsid(S194L) ProteinSARS-CoV-2 Alpha/beta(B.1.1.7/ B.1.351/ A.2.2) Nucleocapsid(P13L) ProteinSARS-CoV-2 Beta(B.1.351/B.1.351.2/b.1.351.3/B.1.427/B.1.429) Nucleocapsid (T205I) ProteinSARS-CoV-2 Gamma(P.1/ P.1.1/ P.1.2) Nucleocapsid (P80R) ProteinSARS-CoV-2 Eta(B.1.525) Nucleocapsid (A12G, T205I) ProteinSARS-CoV-2 (B.1.617) Nucleocapsid (D377Y) ProteinSARS-CoV-2 (B.1.617) Nucleocapsid (R203M, D377Y) ProteinSARS-CoV-2 Delta(B.1.617.2) Nucleocapsid (D63G, R203M, D377Y) ProteinSARS-CoV-2 (B.1.617.3) Nucleocapsid (P67S, R203M, D377Y) ProteinNo cross-reactivity in ELISA with:MERS-CoV Nucleocapsid proteinHCoV-229E Nucleocapsid proteinHCoV-NL63 Nucleocapsid proteinHCoV-HKU1(isolate N5) Nucleocapsid proteinHCoV-OC43 Nucleocapsid protein
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for SARS Nucleocapsid Protein Antibody (001)
Western Blot: SARS Nucleocapsid Protein Antibody (001) [NBP2-90988]
Western Blot: SARS-CoV-2 Nucleocapsid Antibody (001) [NBP2-90988] - 1:2000 dilution. Lane A: SARS-CoV-2 NP Protein (30ng). Lane B: SARS-CoV NP Protein (5ng). Lane C: SARS-CoV-2 (2019-nCoV) NP Protein (30ng). Lane D: SARS-CoV-2 (2019-nCoV) NP Protein (5ng). Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions.Immunocytochemistry/ Immunofluorescence: SARS Nucleocapsid Protein Antibody (001) [NBP2-90988]
Immunocytochemistry/Immunofluorescence: SARS Nucleocapsid Protein Antibody (001) [NBP2-90988] - Nucleocapsid overexpressed HEK293 Cells were stained with purified anti-SARSNucleocapsid Rabbit Mab,then a HRPconjugated second step antibodyImmunocytochemistry/ Immunofluorescence: SARS Nucleocapsid Protein Antibody (001) [NBP2-90988]
Immunocytochemistry/Immunofluorescence: SARS Nucleocapsid Protein Antibody (001) [NBP2-90988] - Analysis of SARS-COV-2 Nucleocapsid overexpressed HEK293 Cells were stained with purified anti-SARS Nucleocapsid Rabbit Mab,then a Alexa Fluor®488-conjugated second step antibody.Applications for SARS Nucleocapsid Protein Antibody (001)
Application
Recommended Usage
ELISA
1:5000-1:10000
Immunocytochemistry/ Immunofluorescence
1:20-1:100
Immunohistochemistry-Paraffin
1:5000-1:20000
Western Blot
1:5000-1:20000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SARS Nucleocapsid Protein
Alternate Names
N, N protein, N structural protein, NC, Nucleocapsid protein, Nucleoprotein, Protein N, SARS coronavirus N protein, SARS coronavirus nucleocapsid protein, SARS CoV, SARS CoV N protein, SARS CoV nucleocapsid protein, SARS N protein, SARS Nucleoprotein, SARSCoV, SARSCoV N protein, SARSCoV nucleocapsid protein, Severe acute respiratory syndrome, SARSCOV2 N protein, SARS-COV-2 nucleocapsid protein, SARS-COV-2 Nucleoprotein
Gene Symbol
N
Additional SARS Nucleocapsid Protein Products
Product Documents for SARS Nucleocapsid Protein Antibody (001)
Product Specific Notices for SARS Nucleocapsid Protein Antibody (001)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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