SARS Nucleocapsid Protein Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56050

Novus Biologicals, part of Bio-Techne
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NB100-56050-0.1mg
NB100-56050SS
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Key Product Details

Species Reactivity

SARS-CoV

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

0.5 mg/ml

View all SARS Nucleocapsid Protein Antibodies »

Product Specifications

Immunogen

The antibody was developed by immunizing rabbits with a synthetic peptide corresponding to amino acids 20-34 (PSDSTGSNQNGERSG) from the N (SARS Nucleocapsid) for the Human SARS coronavirus (Genbank accession no. YP_009724397.2)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: COVID-19 (71%)

Specificity

The was tested on a human cell line transfected with full-length SARS Nucleocapsid cDNA with a predicted molecular weight of 46 kDa.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SARS Nucleocapsid Protein Antibody - BSA Free

Western Blot: SARS Nucleocapsid Protein AntibodyBSA Free [NB100-56050]

Western Blot: SARS Nucleocapsid Protein AntibodyBSA Free [NB100-56050]

Western Blot: SARS Nucleocapsid Protein Antibody [NB100-56050] - Analysis of SARS Nucleocapsid in (A) transfected mouse melanoma cell lysate and (B) untransfected cell lysate using this antibody.

Applications for SARS Nucleocapsid Protein Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Western Blot

1:100-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SARS Nucleocapsid Protein

It has recently been shown that SARS is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness in humans, such as the common cold. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding of the viral spike protein, a 139-kDa protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The most prominent protein in the culture supernatants infected with SARS virus is a 46 kDa nucleocapsid protein. This suggests that the nucleocapsid protein is a major immunogen that may be useful for early diagnostics.

Alternate Names

N, N protein, N structural protein, NC, Nucleocapsid protein, Nucleoprotein, Protein N, SARS coronavirus N protein, SARS coronavirus nucleocapsid protein, SARS CoV, SARS CoV N protein, SARS CoV nucleocapsid protein, SARS N protein, SARS Nucleoprotein, SARSCoV, SARSCoV N protein, SARSCoV nucleocapsid protein, Severe acute respiratory syndrome

Gene Symbol

N

Additional SARS Nucleocapsid Protein Products

Product Documents for SARS Nucleocapsid Protein Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SARS Nucleocapsid Protein Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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