SARS Nucleocapsid Protein Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44205

Novus Biologicals, part of Bio-Techne
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NBP2-44205-100ug
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Key Product Details

Species Reactivity

Validated:

SARS-CoV

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

LYOPH mg/ml

View all SARS Nucleocapsid Protein Antibodies »

Product Summary for SARS Nucleocapsid Protein Antibody

Immunogen

This SARS Nucleocapsid Protein Antibody was developed by immunizing rabbits with a full-length recombinant protein from the N (SARS Nucleocapsid) for the Human SARS coronavirus (Uniprot#: P59595)

Reactivity Notes

SARS Coronavirus. Immunogen displays the following percentage of sequence identity for non-tested species: COVID-19 (91%)

Specificity

The product was purified from monospecific antiserum by protein A affinity purification.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

The recombinant nucleocapsid protein was prepared as follows: SUMO-Nucleocapsid fusion was expressed in E.coli in LB medium and purified using Ni-NTA resin (Qiagen) affinity chromatography. After the fusion was cleaved by the SUMO Protease, the SUMO tag and protease were subtracted from the nucleocapsid using MAC and the nucleocapsid was finally purified using Cation Exchange Chromatography with the Macro-Prep High S resin (BioRad) and size exclusion chromatography.
NBP2-44205 is manufactured exclusively by Rockland Immunochemicals.

This protein A purified antibody is directed against SARS Coronavirus Nucleocapsid (N) protein. The product was purified from monospecific antiserum by protein A affinity purification

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for SARS Nucleocapsid Protein Antibody

Western Blot: SARS Nucleocapsid Protein Antibody [NBP2-44205]

Western Blot: SARS Nucleocapsid Protein Antibody [NBP2-44205]

Western Blot: SARS Nucleocapsid Protein Antibody [NBP2-44205] - Western blot using Protein A Purified anti-SARS CoV Nucleocapsid (N) protein antibody shows detection of a 46 kDa band corresponding to the protein. Approx. 100 ng of protein was loaded for SDS-PAGE and transferred onto nitrocellulose. The blot was incubated with a 1:5,000 dilution of the antibody at room temperature for 1 h followed by detection using IRDy(TM) 800 labeled Goat-a-Rabbit IgG [H&L] (611-132-122) diluted 1:10,000. The fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
Immunocytochemistry/ Immunofluorescence: SARS Nucleocapsid Protein Antibody [NBP2-44205]

Immunocytochemistry/ Immunofluorescence: SARS Nucleocapsid Protein Antibody [NBP2-44205]

Immunocytochemistry/Immunofluorescence: SARS Nucleocapsid Protein Antibody [NBP2-44205] - Immunofluorescence assay using Rabbit Anti-SARS-CoV Nucleocapsid (N) Antibody, showing viral protein synthesis. VeroE6 cells were infected with rSARS-CoV-2. Mock: non-infected cells. At 48 h.p.i, cells were fixed and prepared for immunofluorescence staining with primary antibodies directed against double-stranded RNA (dsRNA) [green] and SARS-CoV Nucleocapsid (N) [red]. Nuclear Counterstain DAPI [blue].
Immunohistochemistry: SARS Nucleocapsid Protein Antibody [NBP2-44205]

Immunohistochemistry: SARS Nucleocapsid Protein Antibody [NBP2-44205]

Immunohistochemistry: SARS Nucleocapsid Protein Antibody [NBP2-44205] - Immunohistochemistry results showing positive staining in placenta tissue using Anti-SARS-CoV Nucleocapsid (N) Antibody.
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Applications for SARS Nucleocapsid Protein Antibody

Application
Recommended Usage

ELISA

1:10000 - 1:50000

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Immunohistochemistry

1:100 - 1:6000

Western Blot

1:2000 - 1:10000
Application Notes
Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 46 kDa in size corresponding to SARS Nucleocapsid (N) protein by western blotting in the appropriate cell lysate or extract. ELISA and lateral flow format has been used to detect virus in extracts from nasal and throat swabs and saliva. IF has been used to determine the presence or absence of virus entering cells especially when This antibody has the ability to neutralize the virus and thereby protect cells from the uptake of live virus. Others have demonstrated the utility of the antibody in flow cytometry studies.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

For the 500 ug size, we recommend reconstituting with 100 ul deionized water. The 0.1 mg size is provided in liquid format (0.02 ml).

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Background: SARS Nucleocapsid Protein

It has recently been shown that SARS is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness in humans, such as the common cold. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding of the viral spike protein, a 139-kDa protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The most prominent protein in the culture supernatants infected with SARS virus is a 46 kDa nucleocapsid protein. This suggests that the nucleocapsid protein is a major immunogen that may be useful for early diagnostics.

Alternate Names

N, N protein, N structural protein, NC, Nucleocapsid protein, Nucleoprotein, Protein N, SARS coronavirus N protein, SARS coronavirus nucleocapsid protein, SARS CoV, SARS CoV N protein, SARS CoV nucleocapsid protein, SARS N protein, SARS Nucleoprotein, SARSCoV, SARSCoV N protein, SARSCoV nucleocapsid protein, Severe acute respiratory syndrome

Gene Symbol

N

Additional SARS Nucleocapsid Protein Products

Product Documents for SARS Nucleocapsid Protein Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SARS Nucleocapsid Protein Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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