SAV1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-13282

Novus Biologicals, part of Bio-Techne
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NBP2-13282
NBP2-13282-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (98%), Rat (98%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SAV1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acids: IGRVAATSLGNLTNHGSEDLPLPPGWSVDWTMRGRKYYIDHNTNTTHWSHPLEREGLPPGWERVESSEFGTYYVDHTNKKAQYRHPCAPSVPRYDQPPPVTYQPQQTERNQSLLVPANPYHTAEIPDWLQVYARAPVKYDHILKWELFQLA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SAV1 Antibody

Western Blot: SAV1 Antibody [NBP2-13282]

Western Blot: SAV1 Antibody [NBP2-13282]

Western Blot: SAV1 Antibody [NBP2-13282] - Analysis in human cell lines U2OS and A-549 using anti-SAV1 antibody. Corresponding SAV1 RNA-seq data are presented for the same cell lines. Loading control: anti-COX4I1.
Immunocytochemistry/ Immunofluorescence: SAV1 Antibody [NBP2-13282]

Immunocytochemistry/ Immunofluorescence: SAV1 Antibody [NBP2-13282]

Immunocytochemistry/Immunofluorescence: SAV1 Antibody [NBP2-13282] - Staining of human cell line SiHa shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: SAV1 Antibody [NBP2-13282]

Immunohistochemistry-Paraffin: SAV1 Antibody [NBP2-13282]

Immunohistochemistry-Paraffin: SAV1 Antibody [NBP2-13282] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.
View 1 more images

Applications for SAV1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SAV1

WW domain-containing proteins are found in all eukaryotes and play an important role in the regulation of a wide variety of cellular functions such as protein degradation, transcription, and RNA splicing. This gene encodes a protein which contains 2 WW domains and a coiled-coil region. It is ubiquitously expressed in adult tissues. The encoded protein is 94% identical to the mouse protein at the amino acid level.

Alternate Names

45 kDa WW domain protein, hWW45, protein salvador homolog 1, salvador homolog 1 (Drosophila), SAV, WW domain-containing, WW45salvador, WWP4,1700040G09Rik

Gene Symbol

SAV1

Additional SAV1 Products

Product Documents for SAV1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SAV1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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