Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI1H10

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all SCAMP2 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human SCAMP2(NP_005688) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

36.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SCAMP2 Antibody (OTI1H10) - Azide and BSA Free

Western Blot: SCAMP2 Antibody (OTI1H10)Azide and BSA Free [NBP2-73999]

Western Blot: SCAMP2 Antibody (OTI1H10)Azide and BSA Free [NBP2-73999]

Western Blot: SCAMP2 Antibody (OTI1H10) - Azide and BSA Free [NBP2-73999] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SCAMP2.
Immunohistochemistry: SCAMP2 Antibody (OTI1H10) - Azide and BSA Free [NBP2-73999]

Immunohistochemistry: SCAMP2 Antibody (OTI1H10) - Azide and BSA Free [NBP2-73999]

Immunohistochemistry: SCAMP2 Antibody (OTI1H10) - Azide and BSA Free [NBP2-73999] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: SCAMP2 Antibody (OTI1H10) - Azide and BSA Free [NBP2-73999]

Immunohistochemistry: SCAMP2 Antibody (OTI1H10) - Azide and BSA Free [NBP2-73999]

Immunohistochemistry: SCAMP2 Antibody (OTI1H10) - Azide and BSA Free [NBP2-73999] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Applications for SCAMP2 Antibody (OTI1H10) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:4000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SCAMP2

Secretory carrier membrane proteins (SCAMPs) are proteins that are components of post-Golgi membranes. These proteins are implicated to function in membrane trafficking. In fibroblasts, SCAMPs are concentrated in compartments that are involved in the recycling of cell surface receptors and endocytosis. In neurons, SCAMPs are associated with synaptic vesicles, secretion granules and transporter vesicles. SCAMPs are composed of four central transmembrane regions and a cytoplasmic tail. Of the five known SCAMPs, SCAMPs 1-3 contain cytoplasmic N-terminal regions with NPF repeats. NPF repeats are found to interact with EH domain proteins that function in budding of transport vesicles from the plasma membrane or the Golgi complex. SCAMPs 4 and 5 lack the N-terminal NPF repeats. SCAMPs 1-4 are all ubiquitously coexpressed while SCAMP 5 is only detectable in the brain. Studies show that SCAMP 5 is expressed late in development which is coincident with expansion of mature synapses.

Alternate Names

secretory carrier membrane protein 2secretory carrier-associated membrane protein 2

Gene Symbol

SCAMP2

Additional SCAMP2 Products

Product Documents for SCAMP2 Antibody (OTI1H10) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SCAMP2 Antibody (OTI1H10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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