SCAMP2 Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-93520

Novus Biologicals, part of Bio-Techne
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NBP2-93520-0.02ml
NBP2-93520-0.1ml
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SCAMP2 Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-152 of human SCAMP2 (NP_005688.2). MSAFDTNPFADPVDVNPFQDPSVTQLTNAPQGGLAEFNPFSETNAATTVPVTQLPGSSQPAVLQPSVEPTQPTPQAVVSAAQAGLLRQQEELDRKAAELERKERELQNTVANLHVRQNNWPPLPSWCPVKPCFYQDFSTEIPADYQRICKML

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SCAMP2 Antibody - Azide and BSA Free

Western Blot: SCAMP2 AntibodyAzide and BSA Free [NBP2-93520]

Western Blot: SCAMP2 AntibodyAzide and BSA Free [NBP2-93520]

Western Blot: SCAMP2 Antibody [NBP2-93520] - Analysis of extracts of various cell lines, using SCAMP2 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 10s.
Immunocytochemistry/ Immunofluorescence: SCAMP2 Antibody - Azide and BSA Free [NBP2-93520]

Immunocytochemistry/ Immunofluorescence: SCAMP2 Antibody - Azide and BSA Free [NBP2-93520]

Immunocytochemistry/Immunofluorescence: SCAMP2 Antibody [NBP2-93520] - Analysis of C6 cells using SCAMP2 at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: SCAMP2 Antibody - Azide and BSA Free [NBP2-93520]

Immunocytochemistry/ Immunofluorescence: SCAMP2 Antibody - Azide and BSA Free [NBP2-93520]

Immunocytochemistry/Immunofluorescence: SCAMP2 Antibody [NBP2-93520] - Analysis of L929 cells using SCAMP2 at dilution of 1:100. Blue: DAPI for nuclear staining.
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Applications for SCAMP2 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SCAMP2

Secretory carrier membrane proteins (SCAMPs) are proteins that are components of post-Golgi membranes. These proteins are implicated to function in membrane trafficking. In fibroblasts, SCAMPs are concentrated in compartments that are involved in the recycling of cell surface receptors and endocytosis. In neurons, SCAMPs are associated with synaptic vesicles, secretion granules and transporter vesicles. SCAMPs are composed of four central transmembrane regions and a cytoplasmic tail. Of the five known SCAMPs, SCAMPs 1-3 contain cytoplasmic N-terminal regions with NPF repeats. NPF repeats are found to interact with EH domain proteins that function in budding of transport vesicles from the plasma membrane or the Golgi complex. SCAMPs 4 and 5 lack the N-terminal NPF repeats. SCAMPs 1-4 are all ubiquitously coexpressed while SCAMP 5 is only detectable in the brain. Studies show that SCAMP 5 is expressed late in development which is coincident with expansion of mature synapses.

Alternate Names

secretory carrier membrane protein 2secretory carrier-associated membrane protein 2

Gene Symbol

SCAMP2

Additional SCAMP2 Products

Product Documents for SCAMP2 Antibody - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SCAMP2 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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