SCAMP4 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-93849
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for SCAMP4 Antibody
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: KVHRIYRGAGGSFQKAQTEWNTGTWRNPPSREAQYNNFSGNSLPEYPTVPSYPGSGQWP
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (87%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SCAMP4 Antibody
Western Blot: SCAMP4 Antibody [NBP1-93849]
Western Blot: SCAMP4 Antibody [NBP1-93849] - Analysis in control (vector only transfected HEK293T lysate) and SCAMP4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).Immunocytochemistry/ Immunofluorescence: SCAMP4 Antibody [NBP1-93849]
Immunocytochemistry/Immunofluorescence: SCAMP4 Antibody [NBP1-93849] - Staining of human cell line A-431 shows localization to plasma membrane, the Golgi apparatus & vesicles.Immunohistochemistry-Paraffin: SCAMP4 Antibody [NBP1-93849]
Immunohistochemistry-Paraffin: SCAMP4 Antibody [NBP1-93849] - Staining of human duodenum shows strong membranous positivity in glandular cells.Applications for SCAMP4 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SCAMP4
Alternate Names
secretory carrier membrane protein 4
Gene Symbol
SCAMP4
Additional SCAMP4 Products
Product Documents for SCAMP4 Antibody
Product Specific Notices for SCAMP4 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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