SCCPDH Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-97630
![Western Blot: SCCPDH Antibody [NBP2-97630]](https://resources.bio-techne.com/images/products/SCCPDH-Antibody-Western-Blot-NBP2-97630-img0002.jpg "Western Blot: SCCPDH Antibody [NBP2-97630]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for SCCPDH Antibody
Immunogen
Produced in rabbits immunized with E. coli-derived Human SCCPDH fragment.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for SCCPDH Antibody
Western Blot: SCCPDH Antibody [NBP2-97630]
Western Blot: SCCPDH Antibody [NBP2-97630] - Lane A: THP-1 Whole Cell LysateLane B: U-87MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:47 kDaObserved band size:47 kDa![Immunocytochemistry/ Immunofluorescence: SCCPDH Antibody [NBP2-97630]](https://resources.bio-techne.com/images/products/SCCPDH-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-97630-img0001.jpg "Immunocytochemistry/ Immunofluorescence: SCCPDH Antibody [NBP2-97630]")
Immunocytochemistry/ Immunofluorescence: SCCPDH Antibody [NBP2-97630]
Immunocytochemistry/Immunofluorescence: SCCPDH Antibody [NBP2-97630] - Immunofluorescence staining of SCCPDH in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human SCCPDH polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.![Immunohistochemistry-Paraffin: SCCPDH Antibody [NBP2-97630]](https://resources.bio-techne.com/images/products/SCCPDH-Antibody-Immunohistochemistry-Paraffin-NBP2-97630-img0003.jpg "Immunohistochemistry-Paraffin: SCCPDH Antibody [NBP2-97630]")
Immunohistochemistry-Paraffin: SCCPDH Antibody [NBP2-97630]
Immunohistochemistry-Paraffin: SCCPDH Antibody [NBP2-97630] - Staining of human SCCPDH in human hepatoma with rabbit polyclonal antibody at 1:100 dilution.Applications for SCCPDH Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:500
Immunohistochemistry-Paraffin
1:50-1:200
Western Blot
1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
PBS
Preservative
0.03% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SCCPDH
Alternate Names
CGI-49, EC 1.5.1.9, FLJ43187, NET11, probable saccharopine dehydrogenase, RP11-439E19.2, saccharopine dehydrogenase (putative)
Gene Symbol
SCCPDH
Additional SCCPDH Products
Product Specific Notices for SCCPDH Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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