SCD-1 Antibody (A9A11-R)

Western Blot: SCD-1 Antibody (A9A11-R) [NBP3-32932]

Western Blot: SCD-1 Antibody (A9A11-R) [NBP3-32932] - Western blot analysis of SCD-1 on different lysates with Mouse anti-SCD-1 antibody (NBP3-32932) at 1/1,000 dilution.

Lane 1: HepG2 cell lysate
Lane 2: HeLa cell lysate
Lane 3: HEK-293 cell lysate
Lane 4: SK-MEL-28 cell lysate
Lane 5: A375 cell lysate
Lane 6: A431 cell lysate
Lane 7: A549 cell lysate

Lysates/proteins at 30 ug/Lane.

Predicted band size: 42 kDa
Observed band size: 37 kDa

Exposure time: 3 minutes 10 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32932) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.