SCD-1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-2476
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-RIKRTGDGNYKSG, from the C Terminus of the protein sequence according to NP_005054.3.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for SCD-1 Antibody
Immunohistochemistry-Paraffin: SCD-1 Antibody [NB100-2476]
Immunohistochemistry-Paraffin: SCD-1 Antibody [NB100-2476] - Staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Flow Cytometry: SCD-1 Antibody [NB100-2476]
Flow Cytometry: SCD Antibody [NB100-2476] - staining of HepG2 cells mixed in with PBL. Detected by fluorescence with PE for SCD in green and with OC515 for CD45 (CYT-450C) in red.Immunohistochemistry-Paraffin: SCD-1 Antibody [NB100-2476]
Immunohistochemistry-Paraffin: SCD Antibody [NB100-2476] - (5ug/ml) staining Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Applications for SCD-1 Antibody
Application
Recommended Usage
Immunohistochemistry
5 ug/ml
Immunohistochemistry-Paraffin
5 ug/ml
Peptide ELISA
detection limit 1:64000
Application Notes
WB: Preliminary experiments gave an approx 30kDa band in Human Adipose, Brain and Liver lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 41.5kDa according to NP_005054.3. The 30kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: In paraffin embedded Human Liver shows textured cytoplam staining in hepatocytes.
Flow Cytometry: Exclusively staining of HepG2 when mixed with Peripheral Blood Lymphocytes.
IHC: In paraffin embedded Human Liver shows textured cytoplam staining in hepatocytes.
Flow Cytometry: Exclusively staining of HepG2 when mixed with Peripheral Blood Lymphocytes.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: SCD-1
Long Name
Stearoyl CoA-desaturase 1, Isoform of the Delta9 Desaturase
Alternate Names
SCD1
Entrez Gene IDs
6319 (Human)
Gene Symbol
SCD
UniProt
Additional SCD-1 Products
Product Documents for SCD-1 Antibody
Product Specific Notices for SCD-1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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