SCD-1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-26393

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all SCD-1 Antibodies »

Product Specifications

Immunogen

Synthetic peptide made to an internal portion of human SCD (within residues 50-100). [Swiss-Prot# O00767]

Localization

endoplasmic reticulum membrane.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SCD-1 Antibody - BSA Free

Western Blot: SCD-1 AntibodyBSA Free [NBP1-26393]

Western Blot: SCD-1 AntibodyBSA Free [NBP1-26393]

Western Blot: SCD Antibody [NBP1-26393] - Normal human liver.
Immunocytochemistry/ Immunofluorescence: SCD-1 Antibody - BSA Free [NBP1-26393]

Immunocytochemistry/ Immunofluorescence: SCD-1 Antibody - BSA Free [NBP1-26393]

Immunocytochemistry/Immunofluorescence: SCD Antibody [NBP1-26393] - SCD staining in Hela cells detected with a Dylight 488 labeled secondary antibody (Green) with Hoechst 33258 nuclear counterstain (Blue).

Applications for SCD-1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50

Western Blot

2 ug/ml
Application Notes
This SCD antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band can be seen at ~41 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SCD-1

SCD (stearoyl-CoA desaturase, or SCD1) is the terminal component of liver microsomal stearyl-CoA desaturase system, that utilizes O2 and electrons from reduced cytochrome b5 to catalyze the insertion of a double bond into a range of fatty acyl-CoA substrates including palmitoyl-CoA and stearoyl-CoA (de novo synthesis of monounsaturated fats from saturated fatty acids). SCD1 promoter region is bound by a wide array of transcription factors, including SREBP-1 and PPAR-alpha and the expression of SREBP-1 as well as SCD1 is upregulated by PI3K/mTOR signaling. Moreover, SCD1 transcription is hormonally upregulated by insulin and growth factors, but inhibited by estrogen and leptin. Oleate (an 18:1 fatty acid) is the major monounsaturated fatty acid of membrane phospholipids, triglycerides, cholesteryl esters, wax esters and alkyl-1,2-diacylglycerol. The ratio of unsaturated to saturated fatty acids is one of the factors influencing membrane fluidity; its alteration is important in a number of diseases, such as cancer, diabetes, obesity, and neurological, vascular and heart disease. This ratio, referred to as Desaturation Index, can be analyzed for multiple fatty acids in plasma and represents a potential biomarker of SCD inhibition.

Long Name

Stearoyl CoA-desaturase 1, Isoform of the Delta9 Desaturase

Alternate Names

SCD1

Entrez Gene IDs

6319 (Human)

Gene Symbol

SCD

UniProt

O00767

Additional SCD-1 Products

Product Documents for SCD-1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SCD-1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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