Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Monkey, Primate

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI5G4

Concentration

0.86 mg/ml

View all SCFD1 Antibodies »

Product Summary for SCFD1 Antibody (OTI5G4)

Immunogen

Full length human recombinant protein of human SCFD1(NP_057190) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for SCFD1 Antibody (OTI5G4)

Western Blot: SCFD1 Antibody (5G4) [NBP2-02031] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-SCFD1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemistry-Paraffin: SCFD1 Antibody (OTI5G4) [NBP2-02031]

Immunohistochemistry-Paraffin: SCFD1 Antibody (OTI5G4) [NBP2-02031]

Immunohistochemistry-Paraffin: SCFD1 Antibody (5G4) [NBP2-02031] - Staining of paraffin-embedded Human pancreas tissue using anti-SCFD1 mouse monoclonal antibody.
Western Blot: SCFD1 Antibody (OTI5G4) [NBP2-02031]

Western Blot: SCFD1 Antibody (OTI5G4) [NBP2-02031]

Western Blot: SCFD1 Antibody (5G4) [NBP2-02031] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SCFD1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SCFD1.
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Applications for SCFD1 Antibody (OTI5G4)

Application
Recommended Usage

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.86 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SCFD1

SCFD1 (sec1 family domain containing 1) belongs to the STXBP-unc/SEC1 family of proteins. SEC1 proteins are involved in synaptic transmission and general secretion. SCFD1 is proposed to play a role in vesicular transport between the endoplasmic reticulum and the Golgi.

Alternate Names

C14orf163, KIAA0917chromosome 14 open reading frame 163, sec1 family domain containing 1, sec1 family domain-containing protein 1, SLY1, SLY1 homolog, sly1p, STXBP1L2RA410, Syntaxin-binding protein 1-like 2, vesicle transport-related protein

Entrez Gene IDs

23256 (Human)

Gene Symbol

SCFD1

Additional SCFD1 Products

Product Documents for SCFD1 Antibody (OTI5G4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SCFD1 Antibody (OTI5G4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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