Scribble Antibody (7C6.D10) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29765

Novus Biologicals, part of Bio-Techne
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NBP2-29765
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 7C6.D10

Format

BSA Free

Concentration

0.5 mg/ml

View all Scribble Antibodies »

Product Summary for Scribble Antibody (7C6.D10) - BSA Free

Immunogen

GST-tagged fusion protein corresponding to the PDZ2 domain of Scribble.

Reactivity Notes

Predicted to cross react with mouse based on sequence homology. Reactivity with other species has not been tested.

Specificity

PDZ2 domain of Scribble.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

220 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Relevant Entrez Gene Numbers: AAL38976

Scientific Data Images for Scribble Antibody (7C6.D10) - BSA Free

Western Blot: Scribble Antibody (7C6.D10)BSA Free [NBP2-29765]

Western Blot: Scribble Antibody (7C6.D10)BSA Free [NBP2-29765]

Western Blot: Scribble Antibody (7C6.D10) - BSA Free [NBP2-29765] - Hela cell lysate was resolved by electrophoresis, transferred to PVDF and probed with anti-Scribble, clone 7C6.D10 (0.1 ug/mL at 1 mg/mL). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP (AP124P) and a chemiluminescence detection system. Arrow indicates Scribble (~220 kDa).

Applications for Scribble Antibody (7C6.D10) - BSA Free

Application
Recommended Usage

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Scribble

Scribble is a tumor suppressor marker first discovered in Drosophila that has been implicated in the control of cell proliferation, polarity, invasion, and metastasis (Humbert, et al, 2003). Mammalian Scribble expresses basolateral staining in epithelial tissue and highly polarized expression in T lymphocytes (Dow, et al, 2003) (Ludford-Menting, et al, 2005), and plays an essential role in the regulation of the polarity specifically involved in directed epithelial migration. Altered expression of human Scribble is associated with invasive epithelial cancers, however, its role in tumour development remains unclear (Dow, et al, 2007).

Alternate Names

CRIB1, protein scribble homolog, SCRB1, SCRIB1, scribbled homolog (Drosophila), Vartul

Gene Symbol

SCRIB

UniProt

Q14160 (Human)

Additional Scribble Products

Product Documents for Scribble Antibody (7C6.D10) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Scribble Antibody (7C6.D10) - BSA Free

Relevant Entrez Gene Numbers: AAL38976

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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