SDHA Antibody (GT20710)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13522

Novus Biologicals, part of Bio-Techne
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NBP3-13522
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (99%), Chicken (97%), Xenopus (94%), Zebrafish (97%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # GT20710

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SDHA Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human SDHA. The exact sequence is proprietary.

Reactivity Notes

Drosophila (87%)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

73 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for SDHA Antibody (GT20710)

Western Blot: SDHA Antibody (GT20710) [NBP3-13522]

Western Blot: SDHA Antibody (GT20710) [NBP3-13522]

Western Blot: SDHA Antibody (GT20710) [NBP3-13522] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [GT20710] (NBP3-13522) diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: SDHA Antibody (GT20710) [NBP3-13522]

Immunocytochemistry/ Immunofluorescence: SDHA Antibody (GT20710) [NBP3-13522]

Immunocytochemistry/Immunofluorescence: SDHA Antibody (GT20710) [NBP3-13522] - SDHA antibody [GT20710] detects SDHA protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SDHA protein stained by SDHA antibody [GT20710] (NBP3-13522) diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:100. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: SDHA Antibody (GT20710) [NBP3-13522]

Immunohistochemistry-Paraffin: SDHA Antibody (GT20710) [NBP3-13522]

Immunohistochemistry-Paraffin: SDHA Antibody (GT20710) [NBP3-13522] - Rat colon. SDHA stained by SDHA antibody [20710] (NBP3-13522) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
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Applications for SDHA Antibody (GT20710)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS, 20% Glycerol

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SDHA

The SDHA gene encodes a major catalytic subunit of succinate-ubiquinone oxidoreductase, a complex of the mitochondrial respiratory chain. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. Mutations

Long Name

SDHA

Alternate Names

CMD1GG, MC2DN1, NDAXOA, PGL5, PPGL5, SDH1, SDH2, SDHF

Gene Symbol

SDHA

Additional SDHA Products

Product Documents for SDHA Antibody (GT20710)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SDHA Antibody (GT20710)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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