SDNSF/MCFD2 Antibody (3A5-G4)
Novus Biologicals, part of Bio-Techne | Catalog # H00090411-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 3A5-G4
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
MCFD2 (AAH40357, 1 a.a. ~ 146 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MTMRSLLRTPFLCGLLWAFCAPGARAEEPAASFSQPGSMGLDKNTVHDQEHIMEHLEGVINKPEAEMSPQELQLHYFKMHDYDGNNLLDGLELSTAITHVHKEEGSEQAPLMSEDELINIIDGVLRDDDKNNDGYIDYAEFAKSLQ
Reactivity Notes
Human. Other species not tested.
Specificity
MCFD2 - multiple coagulation factor deficiency 2
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for SDNSF/MCFD2 Antibody (3A5-G4)
![Western Blot: SDNSF/MCFD2 Antibody (3A5-G4) [H00090411-M01]](https://resources.bio-techne.com/images/products/SDNSF-MCFD2-Antibody-3A5-G4-Western-Blot-H00090411-M01-img0012.jpg "Western Blot: SDNSF/MCFD2 Antibody (3A5-G4) [H00090411-M01]")
Western Blot: SDNSF/MCFD2 Antibody (3A5-G4) [H00090411-M01]
Western Blot: SDNSF/MCFD2 Antibody (3A5-G4) [H00090411-M01] - Analysis of MCFD2 expression in transfected 293T cell line by MCFD2 monoclonal antibody (M01), clone 3A5-G4.Lane 1: MCFD2 transfected lysate(16.4 KDa).Lane 2: Non-transfected lysate.![Immunohistochemistry-Paraffin: SDNSF/MCFD2 Antibody (3A5-G4) [H00090411-M01]](https://resources.bio-techne.com/images/products/SDNSF-MCFD2-Antibody-3A5-G4-Immunohistochemistry-Paraffin-H00090411-M01-img0008.jpg "Immunohistochemistry-Paraffin: SDNSF/MCFD2 Antibody (3A5-G4) [H00090411-M01]")
Immunohistochemistry-Paraffin: SDNSF/MCFD2 Antibody (3A5-G4) [H00090411-M01]
Immunohistochemistry-Paraffin: SDNSF/MCFD2 Antibody (3A5-G4) [H00090411-M01] - Analysis of monoclonal antibody to MCFD2 on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma. Antibody concentration 3 ug/ml.![Western Blot: SDNSF/MCFD2 Antibody (3A5-G4) [H00090411-M01]](https://resources.bio-techne.com/images/products/SDNSF-MCFD2-Antibody-3A5-G4-Western-Blot-H00090411-M01-img0009.jpg "Western Blot: SDNSF/MCFD2 Antibody (3A5-G4) [H00090411-M01]")
Western Blot: SDNSF/MCFD2 Antibody (3A5-G4) [H00090411-M01]
Western Blot: SDNSF/MCFD2 Antibody (3A5-G4) [H00090411-M01] - Analysis of MCFD2 over-expressed 293 cell line, cotransfected with MCFD2 Validated Chimera RNAi ( Cat # H00090411-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MCFD2 monoclonal antibody (M01), clone 3A5-G4 (Cat # H00090411-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.Applications for SDNSF/MCFD2 Antibody (3A5-G4)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Knockdown Validated
Optimal dilutions of this antibody should be experimentally determined.
Sandwich ELISA
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactive against recombinant protein for Western Blot. It has also been used for immunohistochemistry (paraffin), ELISA and RNAi validation.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: SDNSF/MCFD2
Long Name
Stem Cell-derived Neuronal Stem/Progenitor Cell Supporting Factor
Alternate Names
MCFD2
Entrez Gene IDs
90411 (Human)
Gene Symbol
MCFD2
OMIM
227 (Human)
UniProt
Additional SDNSF/MCFD2 Products
Product Documents for SDNSF/MCFD2 Antibody (3A5-G4)
Product Specific Notices for SDNSF/MCFD2 Antibody (3A5-G4)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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