SEC23A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34842

Novus Biologicals, part of Bio-Techne
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NBP2-34842
NBP2-34842-0.025mg
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Hamster

Cited:

Mouse

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

View all SEC23A Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to residues M(1) T T Y L E F I Q Q N E E R D G V R (18) C of rat COPII.

Reactivity Notes

Hamster reactivity reported in scientific literature (PMID: 6452637).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SEC23A Antibody

Western Blot: SEC23A Antibody [NBP2-34842]

Western Blot: SEC23A Antibody [NBP2-34842]

Western Blot: SEC23A Antibody [NBP2-34842] - Analysis was performed whole cell extracts and tissue extracts of HeLa (Lane 1), PC-12 (Lane 2), NIH/3T3 (Lane 3), Mouse Liver (Lane 4) and Rat Liver (Lane 5).
Immunocytochemistry/ Immunofluorescence: SEC23A Antibody [NBP2-34842]

Immunocytochemistry/ Immunofluorescence: SEC23A Antibody [NBP2-34842]

Immunocytochemistry/Immunofluorescence: SEC23A Antibody [NBP2-34842] - Analysis of COP II (green) showing staining in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left).
Flow Cytometry: SEC23A Antibody [NBP2-34842]

Flow Cytometry: SEC23A Antibody [NBP2-34842]

Flow Cytometry: SEC23A Antibody [NBP2-34842] - Analysis of COP II was done on PC-12 cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton (R) X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with COP II Rabbit Polyclonal Antibody (PA1-069A, red histogram) or with rabbit isotype control (pink histogram) at 3-5 ug/million cells in 2.5% BSA.
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Applications for SEC23A Antibody

Application
Recommended Usage

Flow Cytometry

3 - 5 ug / 10^6 cells

Immunocytochemistry/ Immunofluorescence

1:10 - 1:200

Immunohistochemistry

1:10-1:500

Immunoprecipitation

1:10 - 1:500

Western Blot

2 ug/ml
Application Notes
Useful in WB. WB: Detects an approx. 85 kDa protein representing COPII from PC-12 cell extract. May be useful in ICC and IP as reported in scientific literature.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS with 1 mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SEC23A

Coatomer proteins are involved in regulating transport between the endoplasmic reticulum (ER) and the Golgi complex and in intra-Golgi transport. There exist two Coatomer-Protein mechanisms (COPI and COPII) and although they have mechanistic parallels, they are molecularly distinct. The COPI coat is comprised of seven subunits (alpha-, beta-, beta'-, gamma-, delta-, epsilon-, and zeta-COP) in a complex called coatomer. Assembly of the coatomer (COPI) onto non-clathrin coated vesicles is regulated by ADP-ribosylation factor (ARF). Vesicle formation, budding, fusion, and disassembly is dependent on GDP-GTP exchange, COPI, and ARF. COPI has been shown to facilitate retrograde intracellular transport from the ER to the Golgi complex. By contrast, COPII facilitates anterograde transport between these subcellular organelles. COPII has been shown to be independently and selectively recruited to the ER relative to COPI subunits.

Alternate Names

CLSD, MGC26267, protein transport protein Sec23A, Sec23 (S. cerevisiae) homolog A, Sec23 homolog A (S. cerevisiae), SEC23-related protein A

Entrez Gene IDs

26958 (Human); 54160 (Mouse); 58847 (Rat)

Gene Symbol

SEC23A

UniProt

B5DFC3 (Rat)

Additional SEC23A Products

Product Documents for SEC23A Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SEC23A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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