SEC24A Antibody (4D9)

Western Blot: SEC24A Antibody (4D9) [H00010802-M01]

Western Blot: SEC24A Antibody (4D9) [H00010802-M01] - The effects of ouabain and low temperature on ER-related chaperones. CFBE/F508del-CFTR cells were treated with 0.1 uM ouabain or 29C (Low-Temp.) for 6, 24, or 48 h, then the cell lysates were analyzed by immunoblotting using the individual antibodies. The bar graph shows quantification of the band intensities for blots expressed as fold change vs. Time 0 h control. Values in the experiments described are presented as means +/- SD (n = 3). Means were tested for statistical significance using a Student's t-test (*P < 0.05). Image collected and cropped by CiteAb from the following publication (https://journal.frontiersin.org/article/10.3389/fphar.2012.00176/abstract), licensed under a CC-BY license.

Sandwich ELISA: SEC24A Antibody (4D9) [H00010802-M01] - Detection limit for recombinant GST tagged SEC24A is 0.1 ng/ml as a capture antibody.

Western Blot: SEC24A Antibody (4D9) [H00010802-M01] -

Western Blot: SEC24A Antibody (4D9) [H00010802-M01] - The effects of ouabain & low temperature on ER-related chaperones. (A) Left panel: Venn diagram of the intersection between ouabain & low temperature 24 h signatures according to a FDR ≤ 0.05. The numbers indicate distinct genes. The probability *P = 0.0001 was calculated using a hyper-geometric random variable. Right panel: Gene Ontology (GO) cellular processes pie chart of 3530 genes in common between ouabain & low temperature 24 h treatments. (B) The total RNA previously extracted for the microarray analysis was tested by real-time PCR using the individual primers for the gene expressions of HSPA8, HSPA1L, HSPA5, SEC24A, & CANX. Data are presented by the fold change of gene expression vs. DMSO control with mean ± SEM of n = 3 (*P < 0.05) (C) CFBE/F508del-CFTR cells were treated with 0.1 μM ouabain or 29°C (Low-Temp.) for 6, 24, or 48 h, then the cell lysates were analyzed by immunoblotting using the individual antibodies. The bar graph shows quantification of the band intensities for blots expressed as fold change vs. Time 0 h control. Values in the experiments described are presented as means ± SD (n = 3). Means were tested for statistical significance using a Student’s t-test (*P < 0.05). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/23060796), licensed under a CC-BY license. Not internally tested by Novus Biologicals.