SEC6 Antibody (4A7)

Novus Biologicals, part of Bio-Techne | Catalog # H00011336-M01

Novus Biologicals, part of Bio-Techne
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H00011336-M01
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Key Product Details

Species Reactivity

Human, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 4A7

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SEC6 Antibodies »

Product Specifications

Immunogen

EXOC3 (NP_009208, 646 a.a. ~ 745 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SGFGEDVDGYCDTIVAVAEVIKLTDPSLLYLEVSTLVSKYPDIRDDHIGALLAVRGDASRDMKQTIMETLEQGPAQASPSYVPLFKDIVVPSLNVAKLLK

Reactivity Notes

Human. Other species not tested.

Specificity

EXOC3 (4A7)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for SEC6 Antibody (4A7)

Western Blot: SEC6 Antibody (4A7) [H00011336-M01]

Western Blot: SEC6 Antibody (4A7) [H00011336-M01]

Western Blot: SEC6 Antibody (4A7) [H00011336-M01] - Analysis of EXOC3 expression in transfected 293T cell line by EXOC3 monoclonal antibody (M01), clone 4A7.Lane 1: EXOC3 transfected lysate(86.845 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: SEC6 Antibody (4A7) [H00011336-M01] - Detection limit for recombinant GST tagged EXOC3 is approximately 3ng/ml as a capture antibody.

Applications for SEC6 Antibody (4A7)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against transfected lysate and recombinant protein for western blot. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SEC6

The protein encoded by this gene is a component of the exocyst complex, a multiple protein complex essential for targeting exocytic vesicles to specific docking sites on the plasma membrane. Though best characterized in yeast, the component proteins and functions of exocyst complex have been demonstrated to be highly conserved in higher eukaryotes. At least eight components of the exocyst complex, including this protein, are found to interact with the actin cytoskeletal remodeling and vesicle transport machinery. The complex is also essential for the biogenesis of epithelial cell surface polarity.

Alternate Names

exocyst complex component 3, Exocyst complex component Sec6, Sec 6 homolog, SEC6, SEC6L1, SEC6-like 1, SEC6-like 1 (S. cerevisiae), Sec6p

Entrez Gene IDs

11336 (Human)

Gene Symbol

EXOC3

OMIM

608186 (Human)

UniProt

NP_009208 (Human)

Additional SEC6 Products

Product Documents for SEC6 Antibody (4A7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SEC6 Antibody (4A7)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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