SEC62 Antibody (PSH0-25)

Western Blot: SEC62 Antibody (PSH0-25) [NBP3-32939]

Western Blot: SEC62 Antibody (PSH0-25) [NBP3-32939] - Western blot analysis of SEC62 on different lysates with Rabbit anti-SEC62 antibody (NBP3-32939) at 1/5,000 dilution.

Lane 1: Hela cell lysate
Lane 2: Hela cell lysate
Lane 3: K562 cell lysate
Lane 4: SH-SY5Y cell lysate
Lane 5: HepG2 cell lysate
Lane 6: 293T cell lysate
Lane 7: Jurkat cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 46 kDa
Observed band size: 56 kDa

Exposure time: 30 seconds;

10% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32939) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.