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SEC63 Antibody (1A8)

Novus Biologicals, part of Bio-Techne | Catalog # H00011231-M04

Novus Biologicals, part of Bio-Techne
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H00011231-M04

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1A8

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

SEC63 (NP_009145.1, 631 a.a. ~ 728 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KSKITHPVYSLYFPEEKQEWWWLYIADRKEQTLISMPYHVCTLKDTEEVELKFPAPGKPGNYQYTVFLRSDSYMGLDQIKPLKLEVHEAKPVPENHPQ

Specificity

SEC63 - SEC63-like (S. cerevisiae) (1A8)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for SEC63 Antibody (1A8)

Western Blot: SEC63 Antibody (1A8) [H00011231-M04]

Western Blot: SEC63 Antibody (1A8) [H00011231-M04]

Western Blot: SEC63 Antibody (1A8) [H00011231-M04] - Analysis of SEC63 expression in transfected 293T cell line by SEC63 monoclonal antibody (M04), clone 1A8. Lane 1: SEC63 transfected lysate (Predicted MW: 36.52 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: SEC63 Antibody (1A8) [H00011231-M04]

Immunocytochemistry/ Immunofluorescence: SEC63 Antibody (1A8) [H00011231-M04]

Immunocytochemistry/Immunofluorescence: SEC63 Antibody (1A8) [H00011231-M04] - Analysis of monoclonal antibody to SEC63 on HeLa cell. Antibody concentration 10 ug/ml
Sandwich ELISA: SEC63 Antibody (1A8) [H00011231-M04] - Detection limit for recombinant GST tagged SEC63 is 1 ng/ml as a capture antibody.

Applications for SEC63 Antibody (1A8)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SEC63

The Sec61 complex is the central component of the protein translocation apparatus of the endoplasmic reticulum (ER) membrane. The protein encoded by this gene and SEC62 protein are found to be associated with ribosome-free SEC61 complex. It is speculated that Sec61-Sec62-Sec63 may perform post-translational protein translocation into the ER. The Sec61-Sec62-Sec63 complex might also perform the backward transport of ER proteins that are subject to the ubiquitin-proteasome-dependent degradation pathway. The encoded protein is an integral membrane protein located in the rough ER. [provided by RefSeq]

Alternate Names

PRO2507, SEC63 homolog (S. cerevisiae), SEC63LERdj2, SEC63-like (S. cerevisiae), translocation protein SEC63 homolog

Entrez Gene IDs

11231 (Human)

Gene Symbol

SEC63

Additional SEC63 Products

Product Documents for SEC63 Antibody (1A8)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SEC63 Antibody (1A8)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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